background and objectives: there is increasing emergence of multidrug resistant pseudomonas aeruginosa (mdrpa) strains and drug resistance is positively-correlated with biofilm-forming ability. since about 10% of p. aeruginosa genome is controlled by quorum sensing (qs), alteration in its antibiotic susceptibility by targeting qs was the focus of the present study. materials and methods : one d...

In this study, microcosms were established to determine the effect of nitrogen (N) and phosphorus (P) on the multidrug resistance and biofilm-forming abilities of Escherichia coli. The expression of biofilm-formation-related genes was detected to establish correlations between genotype and phenotype. Different concentrations of N and P were added to make one control group and four treatment gro...

We investigated biofilm formation and time of vancomycin (VCM) resistance expression after adhesion to a metal surface in Staphylococcus epidermidis. Biofilm-forming Staphylococcus epidermidis with a VCM MIC of 1 μg/mL was used. The bacteria were made to adhere to a stainless steel washer and treated with VCM at different times and concentrations. VCM was administered 0, 2, 4, and 8 hours after...

Urine specimens were collected aseptically and inoculated directly onto CLED MacConkey agar medium incubated overnight. Single target colonies sub cultured, identification was based on culture, morphology, biochemical characteristics. Enterococci isolates subjected to biofilm assay (Tube method), resistance pattern determined for both biofilm-formers non-biofilm-formers. Genomic DNA extracted u...

In clinical staphylococci, the presence of the ica genes and biofilm formation are considered important for virulence. Biofilm formation may also be of importance for survival and virulence in food-related staphylococci. In the present work, staphylococci from the food industry were found to differ greatly in their abilities to form biofilms on polystyrene. A total of 7 and 21 of 144 food-relat...

Background Pseudomonas is one of the predominant microorganisms of chronic lung infections. Bacteria growing in biofilm often develop multicellular, three-dimensional structures known as micro colonies. Pseudomonas colonizes the lungs by forming biofilm micro colonies throughout the lung. In this study, using Pseudomonas spp. artificial biofilm was grown on flow cell chamber and images were tak...

BACKGROUND Biofilm formation is a major virulence factor contributing to the chronicity of infections. To date few studies have evaluated biofilm formation in infecting isolates of patients including both Gram-positive and Gram-negative multidrug-resistant (MDR) species in the context of numerous types of infectious syndromes. Herein, we investigated the biofilm forming capacity in a large coll...

BACKGROUND AND OBJECTIVE Candida (C.) albicans infection in its biofilm mode of growth has taken centre point with the increasing recognition of its role in human infections due to the development of resistance to the commonly used antibiotic or phenotypic adaptation within the biofilm. Hence, in this study the inhibitory effect of methanol extract of Cassia (C.) spectabilis leaves was evaluate...

Methicillin-resistant Staphylococcus aureus (MRSA) is one of the most threatening pathogens due to its multi-drug resistance (MDR) and strong biofilm-forming capacity. Here, we described the screening of a novel chimeolysin (ClyF) that was active against planktonic and biofilm MRSA. Biochemical tests showed that ClyF was active against all S. aureus clinical isolates tested under planktonic and...