resistance to 6-thioguanine (6-TG) of some cell lines resistant to 6-mercaptopurine (6-MP) have led some investigators to assume that 6-TG and 6-MP havesimilar or identicalmetaboliceffects. This cross-resistance can probably be explained on the basis of the resistance mechanism, which has been so thoroughly investigated by Brock man and his associates (2). Since it now ap pears that both 6-MP a...

MIRJANA POPOVSKA1, VERA RADOJKOVA-NIKOLOVSKA1, ANA MINOVSKA2*, DORIANA AGOP FORNA3, ILIJANA MURATOVSKA1, NORINA CONSUELA FORNA4 1 St. Cyril and Methodius University, Faculty of Dentistry, Department of Oral Pathology and Periodontology, 17 Vodnjanska St. 1000 Skopje, Republic of Macedonia 2 University “Gotse Delchev” of Shtip, Faculty of Medical Sciences, Department of Studies of Dental Medicin...

A large amount of gamma-aminobutyric acid (GABA) was found to accumulate in tomato (Solanum lycopersicum) fruits before the breaker stage. Shortly thereafter, GABA was rapidly catabolized after the breaker stage. We screened the GABA-rich tomato cultivar 'DG03-9' which did not show rapid GABA catabolism after the breaker stage. Although GABA hyperaccumulation and rapid catabolism in fruits is w...

Mathematical simulation of biological processes is a big challenge in both of biology and computer science. This enables biologists to approach the mechanism of life as systems, however, it’s an extremely computation intensive tasks. We’ve developed an FPGA-based hardware accelerator for biochemical simulations. This accelerator has a dedicated pipeline to solve ODEs by its dedicated pipeline m...

Amino acid imbalances refer to the deleterious effects that occur when a second-limiting amino acid or mixture of amino acid lacking a particular limiting amino acid is supplemented in diets marginal in one or more indispensable amino acids. In spite of variation in the conditions that have been used to induce amino acid imbalances, such as protein level in the diet, the extent of difference in...

All cells undergo rapid remodeling of their actin networks to regulate such critical processes as endocytosis, cytokinesis, cell polarity, and cell morphogenesis. These events are driven by the coordinated activities of a set of 20 to 30 highly conserved actin-associated proteins, in addition to many cell-specific actin-associated proteins and numerous upstream signaling molecules. The combined...

RNA interference (RNAi) is an evolutionarily conserved, sequence-specific gene silencing pathway found in eukaryotes, in which 21-nucleotide, small interfering RNAs (siRNAs) guide destruction of a corresponding target mRNA. RNAi is a natural mechanism for both genome surveillance and gene regulation. Moreover, siRNAs can be transfected into cultured mammalian cells, causing the sequence-specifi...

Seed dormancy and resistance to decay are fundamental survival strategies, which allow a population of seeds to germinate over long periods of time. Seeds have physical, chemical, and biological defense mechanisms that protect their food reserves from decay-inducing organisms and herbivores. Here, we hypothesize that seeds also possess enzyme-based biochemical defenses, based on induction of th...

Organisms coordinate biological activities into daily cycles using an internal circadian clock. The circadian oscillator proteins KaiA, KaiB, and KaiC are widely believed to underlie 24-h oscillations of gene expression in cyanobacteria. However, a group of very abundant cyanobacteria, namely, marine Prochlorococcus species, lost the third oscillator component, KaiA, during evolution. We demons...