A xylanase gene, xyn7c, was cloned from Paenibacillus sp. 12-11, an alkalophilic strain isolated from the alkaline wastewater sludge of a paper mill, and expressed in Escherichia coli. The full-length gene consists of 1,296 bp and encodes a mature protein of 400 residues (excluding the putative signal peptide) that belongs to the glycoside hydrolase family 10. The optimal pH of the purified rec...

Saccharomonospora viridis is a thermophilic actinomycete that may have biotechnological applications because of its dye decolorizing activity, though the enzymatic oxidative system responsible for this activity remains elusive. Bioinformatic analysis revealed a DyP-type peroxidase gene in the genome of S. viridis DSM 43017 with sequence similarity to peroxidase from dye-decolorizing microbes. T...

Laccases are multi-copper oxidases that widely distributed in plants and fungi. These enzymes catalyze oxidation of various compounds including phenolics and non-phenolics, and have been used in many industrial processes such as paper industry, biobleaching and bioremediation. A full length cDNA (1767 bp) encoding a putative laccase (Lac) from Rigidoporus vinctus was cloned by polymerase chain ...

Our work on the potential of oxidative biocatalysts in eucalypt biorefinery, including cellulose and biofuel production, is revised in the context of related studies. Organisms selectively degrading lignin from eucalypt wood are ideal models for these biotechnological applications. Among them, the white-rot fungus Ceriporiopsis subvermispora has been largely investigated for eucalypt "biopulpin...

The heterogeneous complexity of sludges and wastewaters has created gross uncertainty and deviations in predictions of suitable models for their measurement. At the same time, it is becoming increasingly obvious that the current paradigms and ideologies are wrought with problems and limitations suggesting the need to move to a more consolidated analytical objective evaluation. Rapid development...

This paper reports high level production of a cellulase-free xylanase using wheat bran, a cost-effective substrate, under submerged fermentation by alkalophilic Bacillus subtilis ASH. Production of xylanase was observed even at alkaline pH up to 11.0 and temperature 60 °C, although the highest enzyme titer was recorded at neutral pH and 37 °C. The enzyme production under optimized fermentation ...

The biobleaching efficiency of xylanase and laccase enzymes was studied on kraft pulps from wood and nonwood based raw materials employed in the Indian paper industry. Treatment of these pulps with xylanase enzyme could result in improved properties, showing 2.0% ISO gain in pulp brightness and/or reducing the demand of chlorine-based bleach chemicals by up to 15% with simultaneous reduction of...