ultrasoundelastographyisanon-invasivemethodwhichimagestheelasticityofsoft-tissues. tomakeanimage, preandafterasmallcompression, ultrasoundradio frequency(rf)signalsareacquiredandthetimedelaysbetweenthemareestimated. thefirstdifferentiationofdisplacementestimationsiscalledelastogram. inthisstudy, wearegoingtomakeanelastogramusingtheprocessingmethodnamed empirical mode decomposition (emd). emdisa...

The molybdate-dependent transcriptional regulator (ModE) from Escherichia coli functions as a sensor of molybdate concentration and a regulator for transcription of operons involved in the uptake and utilization of the essential element, molybdenum. We have determined the structure of ModE using multi-wavelength anomalous dispersion. Selenomethionyl and native ModE models are refined to 1. 75 a...

Molecular modelling studies were performed on some previously reported novel quinoxaline-2-carboxamide 1,4-di-N-oxide derivatives (series 1-9). Using the LigandScout program, a pharmacophore model was developed to further optimize the antimycobacterial activity of this series of compounds. Using the Dock6 program, docking studies were performed in order to investigate the mode of binding of the...

interactions of cationic tetrakis (n,n´,n´´,n´´´-tetramethyltetra-3,4-pyridinoporphyrazinatocobalt(ii) (co(tmtppa)) with synthetic polynucleotides, poly(a-t), poly(g-c) and calf thymus dna have been characterized in 5 mm phosphate buffer, ph 7.2, by optical absorption and fluorescence spectroscopy. the appearance of hypochromicity effect and the red shift in uv-vis spectrum of porphyrazine was ...

Cell division cycle protein 45 (Cdc45) is an essential component of the eukaryotic replicative DNA helicase. We found that human Cdc45 forms a complex with the single-stranded DNA (ssDNA) binding protein RPA. Moreover, it actively loads RPA onto nascent ssDNA. Pull-down assays and surface plasmon resonance studies revealed that Cdc45-bound RPA complexed with ssDNA in the 8-10 nucleotide binding...

The Escherichia coli single-strand binding (SSB) protein is an essential protein involved in DNA replication, recombination, and repair processes. The tetrameric protein binds to ss nucleic acids in a number of different binding modes in vitro. These modes differ in the number of nucleotides occluded per SSB tetramer and in the type and degree of cooperative complexes that are formed with ss DN...

It is becoming increasingly clear that careful treatment of water molecules in ligand-protein interactions is required in many cases if the correct binding pose is to be identified in molecular docking. Water can form complex bridging networks and can play a critical role in dictating the binding mode of ligands. A particularly striking example of this can be found in the ionotropic glutamate r...

Each year, about six million tons of lactose are generated from liquid whey as industrial byproduct, and optimally this large carbohydrate waste should be used for the production of value-added products. Trametes multicolor pyranose 2-oxidase (TmP2O) catalyzes the oxidation of various monosaccharides to the corresponding 2-keto sugars. Thus, a potential use of TmP2O is to convert the products f...

A topological substructural molecular design approach (TOPS-MODE) has been used to formulate structural rules for binding of substrates of P-glycoprotein (P-gp). We first review some of the models developed in the recent literature for predicting binding to P-gp. Then, we develop a model using TOPS-MODE, which is able to identify 88.4% of substrates and 84.2% of non-substrates. When the model i...

in this study, aggregation behavior of a water soluble cationic metalloporphyrin, meso-tetrakis (2,3,5,6-tetrafluoro-n,n´,n´´-trimethyl ammonium phenyl) porphyrin nickel(ii) [ni(ii)(tf4tmapp)]4+ is investigated in 5 mm aqueous phosphate buffer of ph 7.0 at 25.0 °c and various ionic strengths using optical absorption and resonance light scattering spectroscopic methods. the results suggest that ...