HIGHLIGHTS Flavonoids potentially target GyrB. Screened flavonoids have good bioavailability and no toxicity. could preferentially interact with GyrB some of them exhibit higher binding affinity towards than novobiocin.

the purification of biomolecules is a necessary step in many biochemical researches. in this regard, developments of convenient, specific and low cost methods of purification are of particular interest. given the human hemoglobin (hb) affinity toward some charged carbohydrates, interaction of this molecule with human chorionic gonadotropin (hcg) which is a glycoprotein hormone containing sialic...

In this study, the binding of amlodipine (a Ca ++ channel Blocker) and arsenic (metalloid) to bovine serum albumin (BSA) was studied by equilibrium dialysis(ED) method in order to have an insight into their binding chemistry to BSA. Free amlodipine concentration was increased due to addition of arsenic which reduced the binding of the compounds to BSA. However, the free fraction was not increa...

Objective(s): Somatostatin receptor-positive neuroendocrine tumors have been targeted using various peptide analogs radiolabeled with therapeutic radionuclides for years. The better biomedical properties of radioantagonists as higher tumor uptake make these radioligands more attractive than agonists for somatostatin receptor-targeted radionuclide therapy. In this study...

Chemical modifications of innate DNA/RNA aptamers facilitate the improvement their function. Herein, we report our modular strategy to manipulate a thrombin-binding DNA aptamer (TBA) improve its anticoagulation activity and binding affinity. A set amino acid conjugates, termed acid–nucleic hybrids or ANHs, was synthesized incorporated into TBA loop sequences. We found that substitutions with hy...

most of the biological phenomena are influenced by intermolecular recognition and interaction. thus, understanding the thermodynamics of biomacromolecule ligand interaction is a very interesting area in biochemistry and biotechnology. one of the most powerful techniques to obtain precise information about the energetics of (bio) molecules binding to other biological macromolecules is isothermal...

this study was carried out to determination of in vitro binding capacity of organic (wheat and barley) and inorganic (perlite) sources for mn, zn, cu, and fe. for this reason wheat and barley were analyzed chemically for moisture, protein, ash, and ether extract, total carbohydrate, ndf, adf, adl, crude fiber, viscosity, cellulose, and total dietary fiber. the in vitro mineral binding capacity ...

dopamine (da) is one of the main catecholamines in the brain and is crucial for movement coordination, endocrine function, reward, mood, memory and emotions. the dopaminergic system is the primary therapeutic target in the treatment of parkinson’s disease (pd), drug addiction and schizophrenia. notwithstanding, dysfunction of central dopaminergic neurotransmission has also been associated to de...