Meningitis occurs when blood-borne pathogens cross the blood-brain barrier (BBB) in a complex interplay between endothelial cells and microbial gene products. We sought to understand the initial response of the BBB to the human meningeal pathogen group B Streptococcus (GBS) and the organism's major virulence factors, the exopolysaccharide capsule and the beta-hemolysin/cytolysin toxin (beta-h/c...

Septic arthritis is a common and feared complication of staphylococcal infections. Staphylococcus aureus produces a number of potential virulence factors including certain adhesins and enterotoxins. In this study we have assessed the roles of cytolytic toxins in the development of septic arthritis by inoculating mice with S. aureus wild-type strain 8325-4 or isogenic mutants differing in the ex...

clostridium perfringens is an important cause of bacterial food poisoning worldwide. the disease is caused by c. perfringens enterotoxin (cpe) encoded by cpe gene. the aim of this research was to identify the different types of c. perfringens and the presence of cpe gene in isolated bacteria from broilers’ meat marketed in retail meat shops of mashhad city in northeastern of iran. after isolati...

Background: Beta thalassemia is a common health problem in Iran especially in Northern provinces. Premarital screening for thalassemia is compulsory in Iran and identification of the carriers is based on primary CBC (Cell Blood Count) and hemoglobin electrophoresis. Silent mutations on β-globin gene have borderline or normal hematological indices that cannot be detected in premarital scree...

Clostridium perfringens strains (type A) isolated from an integrated poultry operation were subtyped using repetitive-element PCR with Dt primers. Isolates were obtained from fecal, egg shell, fluff, and carcass rinse samples as part of a previously reported temporally linked epidemiological survey. A total of 48 isolates of C. perfringens were obtained from different stages of the broiler chic...

Two subtypes of beta-adrenoceptors, beta 1 and beta 2, mediate cardiac catecholamine effects. These two types differ qualitatively, e.g., regarding G protein coupling and calcium channel stimulation. Transgenic mice overexpressing human beta 2-adrenoceptors survive high-expression levels, unlike mice overexpressing beta 1-adrenoceptors. We examined the role of inhibitory Gi proteins, known to b...

in this study, accumulation of h2o2, malondialdehyde (mda) (as cold-induced oxidative stress indicators), the transcript levels of dehydrin and beta-glucosidase genes (involved in metabolic responses) was evaluated in chickpea cv. jam, using qrt-pcr during control, cold acclimation (ca), cold stress (cs), recovery, and freezing phases. results showed the existence of wide range of genetic capac...

background pseudomonas aeruginosa is a leading cause of infections especially in patients with weakened immune systems. this pathogen is intrinsically resistant to many antibacterial agents. objectives the aim of this study was to survey the antibiotic resistance pattern and frequency of bla oxa10 gene in carbapenemase producing p. aeruginosa isolated from patients in isfahan hospitals. materia...

Strains of Corynebacterium ulcerans and Corynebacterium pseudotuberculosis (Corynebacterium ovis) were examined for the production of diphtheria toxin. A majority of C. ulcerans strains (25 of 37) and 1 C. pseudotuberculosis strain (1 of 14) gave a positive Elek test for diphtheria toxin, and for all strains but 1, production of diphtheria toxin was inhibited at the same level of Fe2+ as was th...