abstract background prevalence of hereditary blood diseases such as sickle cell anemia, sickle thalassemia and thalassemia major are high in khuzestan province. sickle cell anemia and beta-thalassemia are predominantly common in iranian arabs. pulmonary complications account for a large proportion of morbidity and mortality in patients with and sickle cell disease. periodic lung function assess...

in spite of their key role in various immunological processes occurring in the endometrium, t cells- especially + subtype- residing in this mucosal tissue, have not been extensively explored. we present here the profile of expressed genes for variable region of chain of t cell receptor (tcr) in normal endometrium as compared to peripheral blood. samples from endometrium were taken from norm...

Integrins can mediate a diverse variety of functions that are regulated by unknown mechanisms. Integrin alpha 1 beta 1 can serve as a receptor for laminin-1 and collagen in certain cell types, but is a receptor for only collagen in others. To examine the molecular basis of this difference in specificity, three cell types were transfected with cDNA for the rat alpha 1 subunit. Following transfec...

The aim of this study was to investigate the effect of the p15 gene combined with Bcr-abl-specific siRNA and STI571 on the proliferation, cell cycle and apoptosis of K562 chronic myeloid leukemia cells. Using the gene sequence results, we amplified the p15 gene from normal peripheral blood by RT-PCR, and constructed a p15-pcDNA3.1 vector. The K562 cell line with G418 resistance was screened, sy...

Background and Aims: This study was aimed to investigate the anti-cancer effect of Isochrysis galbana microalgae extract. Methods: In study, chemical composition extract analyzed, its cytotoxic against K562, HL60, U937, MOLT-4, Raji cancer cells investigated. ECV304 endothelial were used as a healthy cell line for understanding selective cytotoxicity. To determine effects extract, an MTT cytoto...

A novel water-soluble paclitaxel complex has been prepared by inclusion complexation with bridged bis(beta-cyclodextrin)s and characterized by means of (1)H NMR, SEM, powder X-ray diffraction patterns, TG-DTA, DSC, FT-IR, and 2D NOESY. The cyclodextrins were able to solubilize paclitaxel to levels as high as 2 mg/mL. Furthermore, the cytotoxicity of the novel complexes was assessed using a K562...

objective(s): β-thalassemia is one of the most common genetic disorders in the world. as one of the promising treatment strategies, fetal hemoglobin (hb f) can be induced. the present study was an attempt to reactivate the γ-globin gene by introducing a gene construct containing klf1 binding sites to the k562 cell line. materials and methods: a plasmid containing a 192 bp sequence with two repe...

Recently (J Pharmacol Exp Ther 261:580, 1992), we have shown that K562 leukemia cells express a calcium-signaling purinoceptor with characteristics of the P2T receptor subtype for adenosine diphosphate (ADP) previously found only in platelets. Because these results suggested that the P2T receptor may be an early marker for megakaryocytic differentiation, we studied whether this calcium-signalin...