Model and Methods Energy Hamiltonian.To extend a structure-based model to systems with multiple basins, we modified the contact map and the dihedral angles. Note that all the residue indexes are rearranged from 1 to 366 in the model. To include the side chain dynamics on the conformational change of enzyme, we developed a coarse-grained model in which each amino acid is represented by one or tw...

adsorption and water treatment of ca (ii) and mg (ii) hardness were investigated via adsorption of metal ions onto commercial polyacrylic acid hydrogel beads as a novel sorbent for metal ions (ca2+, mg2+) removal and water treatment. batch equilibrium technique was carried out under the influence of solution ph, contact time, sorbent dosage, initial metal concentration and competitive study. th...

Isolation and purification of biomolecules (doubleand singlestranded DNA, total RNAs, mRNAs, miRNA, proteins), as well as specific cells and organs, facilitate a wide array of downstream applications in gene and protein expression studies, cloning, transfection, protein-protein interactions, immunology, clinical diagnostics, cDNA library synthesis, PCR and qPCR, Sanger and NGS sequencing, and m...

METHODS. Seventeen patients with diagnosed ocular sarcoidosis, 28 with suspected ocular sarcoidosis, 16 with Behçet’s disease, 17 with Vogt-Koyanagi-Harada disease, and 18 healthy subjects were studied. Serum levels of CCL2, CCL5, CXCL8, CXCL9, and CXCL10 were simultaneously measured by cytometric bead array using flow cytometer. In addition, serum CXCL9 and CXCL10 levels in the patients with d...

We present a novel centrifugal microfluidic platform for the highly efficient manipulation and analysis of particles for applications in bead-based assays. The platform uses an array of geometrical V-cup barriers to trap particles using stopped-flow sedimentation under highly reproducible hydrodynamic conditions. The impact parameters governing the occupancy distribution and capture efficiency ...