background: sustainable and commercial production of taxol as an anti cancer drug is a critical point to its clinical application. nowadays, hazel because of rapid growth and wide range distribution is considered as an alternative source of taxol. objective: to increase taxol production the cdna encoding 3-hydroxy-3-methylglutaryl-coa reductase (hmgr) from iranian hazel (genebank accession numb...

Studies pertaining to the detection, isolation anid chemical characterization of naturally occurring and synthetic cytokinins have been aided by the utilization of cultured callus tissues derived from soybean cotyledons (6, 9, 10, 11, 12) and tobacco stem pith (7, 8, 13, 14). Both tissues, when exposed to a specific anid defined chemical milieu, require kinetin or another similarly active 6-sub...

Zygotic embryos excised from immature green fruits of the rattan palm, Calamus thwaitesii and cultured for 16 weeks under optimum culture conditions in Murashige and Skoog (MS) medium supplemented with 31.67 μM 2,4-dichlorophenoxyacetic acid (2,4-D) and 35.23 μM 2,4,5-trichlorophenoxyacetic acid (2,4,5-T) produced mixed (compact and friable) calli at 70 and 92 % rates. The semi-friable part of ...

An efficient protocol was developed to control excessive phenolic compound secretion during callus culture of cotton. As cotton is naturally rich in phenolic compounds factors influencing the phenolic compound secretion, callus induction and proliferation were optimized for getting high frequency callus culture. Different carbon sources such as fructose, glucose, sucrose and maltose were tested...

Plants can regenerate from a variety of tissues on culturing in appropriate media. However, the metabolic shifts involved in callus formation and shoot regeneration are largely unknown. The metabolic profiles of callus generated from tomato (Solanum lycopersicum) cotyledons and that of shoot regenerated from callus were compared with the pct1-2 mutant that exhibits enhanced polar auxin transpor...

Background: The microspores of five tropical japonica and indica rice genotypes were subjected to androgenic studies. effect growth regulators on callus induction studied improve the anther culture efficiency. Methods: cold pre-treatment panicles at 10°C done different days intervals viz., 5, 8, 10 12 days. uninucleate stage selected dusted after pre-treatment. anthers cultured in N6 basal medi...

A simple and reliable protocol for plant regeneration from petioles of micropropagated plants of kiwifruit [Actinidia deliciosa (A. Chev) Liang and Ferguson, var. deliciosa ‘Hayward’] is described. Morphogenic callus was initiated by culturing petioles taken from in vitro-propagated plants. From the media tested, Cheng’s K(h) medium plus 0.1 μM IAA, 4.5 μM zeatin, and 2% sucrose was the best fo...

Agapanthus praecox has become a burgeoning variety in the flower market due to its high ornamental value with unique large blue-purple inflorescence. For rapid entering into market, tissue culture technology or organogenesis an attractive application over conventional reproduction approach. In this study, highly efficient protocol based on indirect been successfully established for A. subsp. or...

context : cumin ( cuminum cyminum l.) as a member of the apiaceae family is one of the most important medicinal plants in the world. objective : an experiment was conducted for evaluation of callus induction optimization in cumin accessions (shahdad, koohbanan, badrood and afghanistan). materials and methods : factorial experiment based on completely randomized design (crd) with three replicati...

We analyzed DNA damage, mitotic activity and polyploidization in Crepis capillaris callus cells during shortand long-term in vitro culture, and the influence of plant growth regulators on these processes. Changes in the concentration of growth regulators altered the stability of callus. The level of DNA damage was highly dependent on the growth regulator composition of the medium. Cytokinin at ...