A novel method has been developed for the selective extraction of DNA from surface-associated bacterial communities from the two model marine benthic algae Ulva australis and Delisea pulchra. The extracted DNA had no detectable contamination with host DNA, was recovered in high yield and quality, and was representative of the bacterial community on the algal surfaces. The DNA is suitable for a ...

We prospectively studied the feasibility and effectiveness of sputum induction in obtaining good quality sputum and its subsequent bacterial yield in children with clinically suspected acute lower-respiratory-tract infection (aLRTI). Good quality sputum was collected in 89/98 (91%) patients. Sputum cultures revealed ≥1 bacterial pathogens in 22 cases (25%). Adverse events were infrequent and mi...

Bacterial expression systems provide economic and logistic advantages in manufacturing proteins for human therapeutic purposes. However, most such proteins accumulate in insoluble biologicaIIy inactive form when overexpressed in bacterial cells. This is the case while attempting to produce recombinant human granulocyte-macrophage colony stimulating factor (hGM-CSF) in E. coli. In this repor...

Analysis of many bacterial genomes is impeded by the inability to separate individual species from complex mixtures of cells or to propagate cells in pure culture. This problem is an obstacle to the study of many bacterial symbionts that live intracellularly in insects and other animals. To recover bacterial DNA from complex samples, we devised a method that facilitates the cloning of DNA fragm...

Pakistan is among top three chickpea producing countries but the crop is usually grown on marginal lands without irrigation and fertilizer application which significantly hampers its yield. Soil fertility and inoculation with beneficial rhizobacteria play a key role in nodulation and yield of legumes. Four kabuli and six desi chickpea genotypes were, therefore, evaluated for inoculation respons...

in order to study the effects of different nutrition systems (organic, chemical and integrated) and biofertilizer (pgpr) on phenology period, grain yield and its component in sunflower (helianthus annuus l.) an experiment was conducted using alestar cultivar at the research farm of college of agriculture, tarbiat modares university in 2007. five levels of nutrition systems including f1 (100% or...

In the past few years, the world has taken a new view to herbal remedies and their applications and many researchers in various fields of medicine are studying this subject. In our program searching for antibacterial agents we have selected an extract prepared from the Pentanema divaricata. No report on the antibacterial activity of this species has been published in literature. Pentanema divar...

In the past few years, the world has taken a new view to herbal remedies and their applications and many researchers in various fields of medicine are studying this subject. In our program searching for antibacterial agents we have selected an extract prepared from the Pentanema divaricata. No report on the antibacterial activity of this species has been published in literature. Pentanema divar...