Background: Patients with fever of unknown origin (FUO) are elusive and challenging clinical cases. Timely detection and identification of blood borne pathogen is one of the most important functions of microbiology laboratory. Objectives: To evaluate the efficacy of BACTEC 9050 in comparison to conventional blood culture for detection of bacterial isolates in clinically suspected cases of fever...

The recently developed ESP Culture System II (AccuMed, Chicago, Ill.) was compared with radiometric BACTEC 460TB (Becton Dickinson, Towson, Md.) and with Lowenstein-Jensen medium for recovery of mycobacteria from over 2,500 clinical specimens both of respiratory and nonrespiratory origin, including blood. The majority of the 219 mycobacterial isolates (129) belonged to the Mycobacterium tubercu...

OBJECTIVE To evaluate available evidence on the diagnostic value of radioactivity-free cultivation and detection technologies for rapid detection of rifampicin resistance in Mycobacterium tuberculosis. METHODS A fully recursive literature search was conducted in PubMed, EMBASE, Biosis, Web of Science (all 1990-2010), CBMWeb (1978-2010), and Google Scholar. QUADAS items were used to evaluate t...

BACKGROUND Infectious diseases are a leading cause of morbidity and mortality in developing countries. The aim of this study was to compare the results of blood culture employing the conventional and BACTEC methods for detection of bacterial infection in Taleghani Hospital, Tehran. MATERIALS AND METHODS This was a descriptive study carried out for 3 months (March 2014-May 2014) on 272 inpatie...

A total of 463 respiratory specimens, all smear positive for acid-fast bacteria, were inoculated onto routine solid media and into BACTEC 7H12 Middlebrook medium for detection of mycobacterial growth. Conventional drug susceptibility testing (1% proportion method) was performed on Middlebrook 7H10/7H11 medium, and radiometric susceptibility testing was performed on BACTEC 7H12 medium. The avera...

The BACTEC 16B resin medium was developed to detect bacteremia in patients receiving antimicrobial therapy. Over a 9-month period, we compared the efficacy of the BACTEC 16B bottle to the conventional aerobic BACTEC 6B bottle. Of a total of 1,524 sets of blood cultures, 79 yielded presumed pathogens. Of these 79 sets, 42 (53.2%) were positive in both the 16B and 6B bottles, 23 (29.1%) were posi...

BACKGROUND For the diagnosis of prosthetic joint infection (PJI) automated BACTEC™ blood culture bottle methods have comparable sensitivity, specificity and a shorter time to positivity than traditional cooked meat enrichment broth methods. We evaluate the culture incubation period required to maximise sensitivity and specificity of microbiological diagnosis, and the ability of BACTEC™ to detec...

In a meta-analysis of 10 studies, the BACTEC 960/MGIT and BACTEC 460 systems showed a sensitivity and specificity in detecting mycobacteria (1,381 strains from 14,745 clinical specimens) of 81.5 and 99.6% and 85.8 and 99.9%, respectively. Combined with solid media, the sensitivity of the two systems increased to 87.7 and 89.7%, respectively.

Pseudobacteremias in blood cultures performed on the BACTEC radiometric blood culture system have been reported. We report three cases of cross contamination with Mycobacterium avium that occurred when mycobacteria were cultured with the BACTEC 460 TB system. Malfunction of the needle sterilization heating block was demonstrated.

At Yale-New Haven Hospital, 46 specimens submitted for mycobacterial culture during an 8-week period in 1989 were positive for Mycobacterium gordonae, a nontuberculous acid-fast bacterium (AFB) of low pathogenicity. The specimens were submitted from 34 patients who came from various inpatient and outpatient services. Four patients were begun on antimycobacterial therapy on the basis of an AFB i...