A cytochrome oxidase was purified 52-fold from membranes of alkalophilic Bacillus firmus RAB by extraction with Triton X-100, ion-exchange and hydroxyapatite chromatography, and gel filtration. On denaturing gels, the purified enzyme dissociated into two subunits of 56,000 and 40,000 Mr as well as a cytochrome c with an Mr of approximately 14,000. Heme contents calculated for an enzyme with a m...

Soil contamination by textile and tannery effluents is a great concern to the environment, Complex having number of dyes accumulate in soil there polluting environments loss microbial species soil. Samples were collected physicochemical analyses was determined. The pH basic with 8.0 10.1 from dyeing site respectively. organic carbon very low 0.76% 0.06%, Nitrogen (0.067% 0,095%), phosphorus 0.8...

The generic position of 14 strains of gram-positive bacteria able to use methanol as a growth substrate was determined. All are obligately aerobic, thermotolerant organisms that are able to grow at temperatures of 35 to 60 degrees C. Nine of the strains produce oval spores at a subterminal-to-central position in slightly swollen rod-shaped cells. DNA-DNA hybridization studies, 5S rRNA sequence ...

Bartonella bacilliformis is the etiologic agent of Carrion's disease. This disease has two well established phases, the most relevant being the so called Oroya Fever, in which B. bacilliformis infect the erythrocytes resulting in severe anemia and transient immunosuppression, with a high lethality in the absence of adequate antibiotic treatment. The presence of B. bacilliformis was studied in 1...

Aspects of the life cycle of the nematomorph Gordius robustus were investigated. Gordius robustus larvae fed to Tenebrio molitor (Coleoptera: Tenebrionidae) readily penetrated and subsequently encysted in the posterior portion of the midgut wall. Parasite development was then arrested in each infected beetle. Upon feeding these cysts to laboratory-reared Gryllus firmus, worm larvae developed to...

Cocoa bean shell (CBS), a by-product of the chocolate industry, has been employed as substrate to obtain poly(3-hydroxybutyrate) (PHB) by fermentation with Bacillus firmus. With this aim, acid-thermal hydrolysis CBS (20% w/v) was conducted at 135 °C for 10 min so that broths rich in fermentable sugars were obtained. These broths, both non-centrifuged and centrifuged, media. Significant polymer ...