نتایج جستجو برای: b3 loci by through specific primers
تعداد نتایج: 7968333 فیلتر نتایج به سال:
The application of commercially available microsatellite primers, developed for genomic analysis of the laboratory rat (Rattus norvegicus) and laboratory mouse (Mus musculus), to the genotyping of wild rodents provides a rapid and inexpensive alternative to cloning, sequencing and synthesizing species-specific primers de novo. The effort required to identify a useful panel of heterologous (inte...
Forensic experts in uncovering the truth of a case must go through identification, documentation, and collection evidence. Sometimes at crime scene, lip prints are found on surface an object such as mouth plastic bottle. Lip print research bottle mouths was carried out for 1, 3 7 days. There 21 samples with details controls, 6 first day, 3rd 7th day. DNA extraction using DNAzol, quantification ...
Lophopyrum elongatum, a close relative of wheat, provides a source of novel genes for wheat improvement. Molecular markers were developed to monitor the introgression of L. elongatum chromosome segments into hexaploid wheat. Existing simple sequence repeats (SSRs) derived from genomic libraries were initially screened for detecting L. elongatum loci in wheat, but only 6 of the 163 markers teste...
dynamic assessment according to vygotskys sociocultural theory, states that instruction and assessment are interrelated. using static forms of assessment with second and foreign language students could do more harm than good. many teachers neglect to take account of the complexity involved in learning a second or foreign language and often wonder why learning of a language take so long. thi...
the kermani sheep is an important meat producing an animal in iran and its economic efficiency is mainly dependent on its growth and reproduction ability. inter-simple sequence repeat (issr) is the genome region between microsatellite loci. the aim of this study was to evaluate the association of two different issr markers with predicted breeding values (pbv) of body weight trait in kermani she...
hydrochlorothiazide (hct) is a diuretic agent which is shown to be effective in the treatment of hypertension. literature reports have demonstrated that urinary excretion data may be used to assess the bioavailability of various formulations containing this thiazide. also hct consumption by the athletes is one of the drugs which should be regulated by world anti-doping agency (wada), because of...
abstract target-oriented approaches to translation studies are regarded as recent theories of translation. one of the most famous theories among these approaches is descriptive translation studies presented by toury (1995). this theory gives a new dimension to translation studies and gives importance to the descriptive rather than prescriptive studies. it also identifies three sets of transla...
We have isolated major histocompatibility complex (MHC) class II beta loci from the little greenbul (Andropadus virens), an African songbird. We utilized preexisting information about conserved regions of the avian MHC to design primers to amplify a pool of sequences representing multiple loci. From this pool, a unique locus spanning 1109 bp that we designate as Anvi-DAB1 was cloned and sequenc...
Background: Splicing by overlap extension (SOE) PCR is used to create mutation in the coding sequence of an enzyme in order to study the role of specific residues in protein’s structure and function. Objectives: We introduced a nested-SOE-PCR (N –SOE-PCR) in order to increase the specificity and generating mutations in a gene by SOE-PCR. Materials and Methods: Genomic DNA from Bacillus thermo...
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