Recombinant avian poxviruses [fowlpox and canarypox (ALVAC)], restricted for replication in nonavian cell substrates and expressing granulocyte/macrophage-colony stimulating factor (avipox-GM-CSF), were evaluated for their ability to enrich an immunization site with antigenpresenting cells (APCs) and, in turn, function as biological vaccine adjuvants. Avipox-GM-CSF administered as a single s.c....

Evidence is presented that poxvirus deoxyribonucleic acid (DNA) synthesis required concurrent protein synthesis. The protein requirement in question can be distinguished from viral-induced thymidine kinase and DNA polymerase by virture of the instability of its messenger ribonucleic acid and its stoichiometric rather than catalytic relation to DNA synthesis. The protein(s) required did accumula...

We examined the safety, immunogenicity and efficacy of a prime-boost vaccination regime involving two poxvirus malaria subunit vaccines, FP9-PP and MVA-PP, expressing the same polyprotein consisting of six pre-erythrocytic antigens from Plasmodium falciparum. Following safety assessment of single doses, 15 volunteers received a heterologous prime-boost vaccination regime and underwent malaria s...

Poxviruses, of which vaccinia is the prototype, are complex DNA viruses that display an unusual degree of physical and genetic autonomy from the host cell. The vaccinia genome encodes most, if not all, of the functions required for three temporally regulated phases of transcription and for DNA replication (Moss 1990a,b, 1994; Traktman 1990a,b, 1991). In addition, the 192-kb genome encodes numer...

We describe the use of Java Pattern Finder (JaPaFi) to identify short (<100 nt) highly conserved sequences in a series of poxvirus genomes. The algorithm utilizes pattern matching to identify approximate matches appearing at least once in each member of a set of genomes; a key feature is that the genomes do not need to be aligned. The user simply specifies the genomes to search, minimum length ...

Poxvirus has been identified morphologically from skin lesions in captive and free-ranging bottlenosed dolphins, Tursiops truncatus and a stranded Atlantic white-sided dolphin, Lagenorhynchus acutus. The lesions, commonly referred to as ring or pinhole lesions, appear as solitary or coalesced circular grey blemishes. Advanced ring lesions may take the form of black punctiform stippled patterns ...

Ubiquitination plays a critical role in many cellular processes. A growing number of viruses have evolved strategies to exploit the ubiquitin-proteasome system, including members of the Poxviridae family. Members of the poxvirus family have recently been shown to encode BTB/kelch and ankyrin/F-box proteins that interact with cullin-3 and cullin-1 based ubiquitin ligases, respectively. Multiple ...

RINGKASAN Contoh-contoh seperti kuping, kutil, najis tengkorok dan paru-paru telah dihantar ke makmal untuk menentukan penyakit-penyakit haiwan dengan menggunakan mikroskop lektron. Pemeriksaan contoh dari kes puru kambing dengan puru ayam telah menunjukkan adanya kumin-kumin berupa bentuk poxvirus. Kumin-kumin berupa bentuk coronavirus telah juga dilihat dari najis anak lembu berpenyakit ceret...

Poxvirus Orthologous Clusters (POCs) is a JAVA client-server application which accesses an updated database containing all complete poxvirus genomes; it automatically groups orthologous genes into families based on BLASTP scores for assessment by a human database curator. POCs has a user-friendly interface permitting complex SQL queries to retrieve interesting groups of DNA and protein sequence...