نتایج جستجو برای: automated sequencer
تعداد نتایج: 130519 فیلتر نتایج به سال:
Next-generation sequencing (NGS) is emerging as a powerful tool for elucidating genetic information for a wide range of applications. Unfortunately, the surging popularity of NGS has not yet been accompanied by an improvement in automated techniques for preparing formatted sequencing libraries. To address this challenge, we have developed a prototype microfluidic system for preparing sequencer-...
Accurate and fast genotyping of single nucleotide polymorphisms (SNPs) is of significant scientific importance for linkage and association studies. We report here an automated fluorescent method we call multiplex automated primer extension analysis (MAPA) that can accurately genotype multiple known SNPs simultaneously. This is achieved by substantially improving a commercially available protoco...
PCR-based setup for high-throughput cDNA library sequencing on the ABI 3700 automated DNA sequencer.
Many large-scale sequencing projects are now being conducted or considered that rely on the latest capillary electrophoresis-based automated sequencing system from Applied Biosystems, the ABI 3700 (PE Biosystems, Foster City, CA, USA). Because of the electrokinetic injection protocol and the physical properties of the capillaries and polymer used, reaction setups that have been optimized for t...
We are investigating approaches to increase DNA sequencing quality. Since a majorfactor in sequence generation is the cost of reagents and sample preparations, we have developed and optimized methods to sequence directly plasmid DNA isolated from alkaline lysis preparations. These methods remove the costly PCR and post-sequencing purification steps but can result in low sequence quality when us...
Constant Denaturant Capillary Electrophoresis (CDCE) is a separation tool based on the cooperative melting equilibrium principle that is used to detect mutations as low as of 106. This technique has already demonstrated invaluable clinical applications in correlated preventative prognosis, medical evaluations, and interventions. Accordingly, there is a high demand to utilize CDCE as a cost-effe...
52 BioTechniques Vol. 32, No. 1 (2002) 8.Joyner, K.L., X.-R. Wang, J.S. Johnston, H.J. Price, and C.G. Williams. 2001. DNA content for Asian pines parallels New World relatives. Can. J. Bot. 79:179-191. 9.Kinlaw, C.S. and D.B. Neale. 1997. Complex gene families in the pine genome. Trends Plant Sci. 2:356-359. 10.Kriebel, H.B. 1985. DNA sequence components of the Pinus strobus nuclear genome. Ca...
Capturing physical data in the context of measurement systems is a demanding process that often requires many repetitions with different settings. To assist in this activity, a domain-specific modeling language (DSML) called Sequencer has been developed to enable the improved definition of measurement procedures. With Sequencer, the level of abstraction has been raised and sophisticated changes...
نمودار تعداد نتایج جستجو در هر سال
با کلیک روی نمودار نتایج را به سال انتشار فیلتر کنید