Two distinctive colony morphologies were noted in a collection of Pseudomonas aeruginosa transposon insertion mutants. One set of mutants formed wrinkled colonies of autoaggregating cells. Suppressor analysis of a subset of these mutants showed that this was due to the action of the regulator WspR and linked this regulator (and the chemosensory pathway to which it belongs) to genes that encode ...

The bio-chemical changes which occur in fish tissues are due to two factors, autolysis and bacterial action. As early as 1903, Muller reported that changes due to autolysis took place when sterile fish tissues were incubated. More recently attention has been focussed on the relative importance of these two causes of bio-chemical change. Reed, Rice and Sinclair (1929) presented evidence that aut...

An analytical procedure for the resolution of trypsin and its derivatives would be useful in searching for active degradation products in tryptic autolysates as well as for other studies of the reactions of trypsin. Analytical procedures are frequently frustrated when applied to this enzyme because of the autolysis which occurs during the analysis. For this reason some previous analyses have be...

Almost all Anatomic Pathology laboratories have experienced that tissue patient who comes to these without adequate fixation and causes complicated diagnosis. In addition, also makes advanced procedures such as immunohistochemical staining or DNA RNA extraction difficult because protein, DNA, become damaged due inadequate fixation. The procedure should be the responsibility of clinicians, speci...

1. Autolysis of Pneumococcus is accompanied by proteolysis, which results in an increase in amino and non-coagulable nitrogen. 2. Autolysis of Pneumococcus is accompanied by lipolysis during which there is a liberation of ether-soluble fatty acids. 3. When extracts containing the active intracellular enzymes are added to heat-killed pneumococci, lysis of the cells occurs and there is an increas...