AIM To assess microbial contamination of 20% autologous serum (AS) eye drops used in a hospital inpatient setting. METHOD 14 patients received autologous serum drops from 4 to 14 days with a cumulative total of 67 days. For each day the first and last drop (total 134 samples) was cultured on broth and blood agar. RESULTS Four patients (9 samples) grew Staphylococcus epidermidis only. One pa...

The normal lymphocytes of 11 of 18 paexpectation of demonstrating a “blocktients with acute leukemia in remission ing” effect of the former and no effect or showed a positive blastogenic response perhaps even facilitation with the latter. in vitro to autologous leukemia cells. The However, this was not the case, and no effect of autologous serum on this response consistent differences in blasto...

Background/Aim: To describe the clinical progress and management of ocular side effects in a 35-year-old patient with metastatic breast cancer who underwent oral chemotherapy capecitabine lapatinib. Materials Methods: Slit lamp evaluation revealed bilateral perikeratic hyperemia, perilimbal conjunctival edema associated corneal marginal infiltrates epithelial anterior stromal defects both eyes....

We have investigated the regulation of self tolerance in mice by examining lymphocyte reactivity in vitro against two subpopulations of autologous testicular cells: germ cells that were derived from the seminiferous tubules, and interstitial somatic cells. In the presence of germ cells, lymphocyte proliferation was strongly reduced. In contrast, somatic interstitial cells stimulated lymphocyte ...

A substantial proportion of human immunodeficiency virus type 1 (HIV-1)-infected individuals has cross-reactive neutralizing activity in serum, with a similar prevalence in progressors and long-term nonprogressors (LTNP). We studied whether disease progression in the face of cross-reactive neutralizing serum activity is due to fading neutralizing humoral immunity over time or to viral escape. I...

OBJECTIVE To evaluate the effectiveness of 20% autologous serum as a treatment for neurotrophic keratopathy. MATERIAL AND METHODS A longitudinal, observational and descriptive study was performed on 19 patients (22 eyes) with neurotrophic keratopathy in different stages of Mackie's classification. The following variables were evaluated on the first visit, and then 4 months later: best correct...

OBJECTIVES To assess microbial contamination of common non-preservative eye drops stored at 4 degrees C and non-preservative fortified antibiotic eye drops used in a hospital inpatient setting. MATERIAL AND METHOD A prospective study of the sterility of non-preservative eye drops was examined by dividing the patients into 2 groups. Group 1 composed of 5 samples of each ophthalmic preparation,...

Mechanisms by which macrophages discriminate between "mature-self" and "senescent-self" were investigated using the human red blood cell (RBC) system as a model. Conditions simulating those encountered in situ were adhered to as closely as possible by using short term culture techniques and incubating with autologous macrophages and immunoglobulins (Ig). It was found that RBC aged in vitro were...

Long-term dietary and pharmacological treatments for obesity have been questioned, particularly in individuals with severe obesity, so a new approach may involve adipose tissue transplants, particularly autologous transplants. Thus, the aim of this study was to evaluate the metabolic effects of autologous subcutaneous adipose tissue (SAT) transplants into two specific intraabdominal cavity site...

AIM To evaluate the efficacy of autologous serum application for the treatment of dry eye in Sjögren's syndrome. METHODS The stability of essential components (EGF, vitamin A, and TGF-beta) in preserved serum were examined following preservation at 4 degrees C and -20 degrees C. In a primary clinical trial, 12 patients with Sjögren's syndrome were treated with autologous serum (diluted to 20%...