In the study, an aqueous two phase system (ATPS) coupled with ultrasound was employed to extract lignans from Zanthoxylum armatum. Three standard lignans, namely (-)-fargesin, sesamin and L-asarinin, were used as marker compounds, and extraction was optimized and projected by response surface methodology (RSM) and artificial neural network (ANN). The optimal condition for ATPS with 20% n-propan...

1 Abstract Researchers of reasoning in computer science and psychology are estranged sib-lings. The tools they use to investigate patterns of inference seldom overlap, because the goals of each group differ. A computer scientist's primary goal is to efficiently engineer systems based on logical calculi. But, human reasoning systematically violates the constraints imposed by orthodox logic. One ...

The lateral-flow immunoassay (LFA) is an inexpensive point-of-care (POC) paper-based diagnostic device with the potential to rapidly detect disease biomarkers in resource-poor settings. Although LFA is inexpensive, easy to use, and requires no laboratory equipment, it is limited by its sensitivity, which remains inferior to that of gold standard laboratory-based assays. Our group is the only on...

The atmospheric temperature profiles (ATPs) retrieved through the geostationary Interferometric Infrared Sounder (GIIRS) onboard FY-4A satellite (GIIRS/FY-4A) can effectively fill gap of scarce conventional sounding data in Taklimakan Desert (TD), second largest desert world, with an area 330,000 square kilometers. In this study, we take experimental radiosonde observations (RAOB) from one RAOB...

In this paper, the efficiency of an aqueous two-phase system (ATPS) for purification of nanometer-sized bioparticles, plasmid DNA (pDNA), was studied. Polymer-salt ATPS consisting of polyethylenglycol (PEG)-K2HPO4 was used for the purification of 7 kb and 14 kb plasmid vectors. PEG-300 and PEG-1450 were applied to investigate the influence of different molecular mass of PEG on partitioning beha...

This paper studies the Rayleigh-Plateau instability for co-flowing immiscible aqueous polymer solutions in a microfluidic channel. Careful vibration-free experiments with controlled actuation of the flow allowed direct measurement of the growth rate of this instability. Experiments for the well-known aqueous two phase system (ATPS, or aqueous biphasic systems) of dextran and polyethylene glycol...

A relationship is proposed for the interfacial partitioning of protein in poly(ethylene glycol) (PEG)-phosphate aqueous two-phase system (ATPS). The relationship relates the natural logarithm of interfacial partition coefficient, ln G to the PEG concentration difference between the top and bottom phases, Δ[PEG], with the equation ln G=AΔ[PEG]+B. Results showed that this relationship provides go...

Aqueous two-phase system (ATPS) is considered a new method to remove heat stable salts (HSS) from flue gas desulfurizer 1,4-bis-(2-hydroxyethyl)-piperazine (BHEP). In this work, the liquid-liquid phase equilibrium data of BHEP-Na2SO4 ATPS in 303.15 – 323.15 K were measured and correlated by NRTL equation with redefined adjustable characteristic constant at different concentrations. It revealed ...

Sulfate assimilation is a critical component of both primary and secondary metabolism. An essential step in this pathway is the activation of sulfate through adenylation by the enzyme ATP sulfurylase (ATPS), forming adenosine 5'-phosphosulfate (APS). Proteobacterial ATPS overcomes this energetically unfavorable reaction by associating with a regulatory G protein, coupling the energy of GTP hydr...