Acidic luminal pH and low [HCO(3)(-)] maintain sperm quiescent during maturation in the epididymis. The vacuolar H(+)-ATPase (V-ATPase) in clear cells is a major contributor to epididymal luminal acidification. We have shown previously that protein kinase A (PKA), acting downstream of soluble adenylyl cyclase stimulation by alkaline luminal pH or HCO(3)(-), induces V-ATPase apical membrane accu...

The in vivo effect of vitamin D on (Ca2+ + Mg2+)ATPase activity was examined in a plasma membrane fraction of rat circulating mononuclear cells (MPM). Although there was no significant difference in the ATPase activities in red blood cell ghosts, (Ca”’ + Mg2+)-ATPase activity in MPM was significantly higher (p < 0.05) in long-term vitamin DB-replete rats (100 IU/day for 6 months) than that in v...

Few studIies have been conducted on adenosine triphosphatase (ATPase) in plant mitochondria (3, 5. 6). ATPase activity is important in plant metabolism since it results in the loss of the energy of ATI , and is therefore closely associated with reactions involving phosphorylation and utilization of or requiremiients for ATP. The ternm ATPase is being use(l lhere as defined by Potter (10) to ind...

Purified perigranular and plasma membranes isolated from rat peritoneal mast cells were examined for Ca2+- and Mg2+-dependent ATPase activity. Isolated perigranular membranes contained only a low-affinity Ca2+- or Mg2+-dependent ATPase (Km greater than 0.5 mM). The plasma membranes contained both a low-affinity Ca2+- or Mg2+-dependent ATPase (Km = 0.4 mM, Vmax. = 20 nmol of Pi/min per mg), as w...

The effects of different concentrations gallic acid (0 mg/g, 1 2 4 and 6 mg/g) on the physicochemical properties Lateolabrax japonicas myofibrillar protein were studied with 400 W ultrasound. results showed that decreased particle size, total sulfhydryl group, carbonyl dimer tyrosine content Ca 2+ -ATPase activity (P&lt;0.05) protein, however, increased zeta potential. Ultrasonic wave could coo...

The responses of sea cucumbers to salinity changes are reflected in the expression profiles multiple genes and microRNAs. miR-2008 Pleckstrin Homology Domain Containing A3 (PLEKHA3) were previously shown be involved cucumbers. To better understand relationship between PLEKHA3, PLEKHA3 predicted interaction was investigated using luciferase reporter assays gene knockdowns. Dual-luciferase analys...

Under conditions where cytochalasin B induces ATPase activity of monomeric actin (0.3 mM MgCl2, 1 mM EGTA, 30 microns cytochalasin B, 1 mM ATP) the rate constant of the exchange of actin-bound epsilon-ATP for free ATP is about 4-6 times faster than steady state ATPase activity. When a stoichiometric ATP-actin complex is extracted with PCA (single turnover experiment) the apparent rate constant ...

The objectives of this study were to determine the influence of pH and MyHC isoforms on myofibrillar and actin-activated myosin subfragment 1 (S1) ATPase activity and the protective effect of actin. Red (RST) semitendinosus and white (WST) semitendinosus myofibrils were incubated at pH 7, 6, or 5.5 with 0 or 2mM ATP. RST and WST S1 isolates were incubated at pH 7, 6, or 5.5 in the presence or a...

To understand how rapid changes in blood pressure can regulate Na-K-ATPase in the kidney cortex, we tested the hypothesis that a short-term (5 min) decrease in renal perfusion pressure will increase the amount of Na-K-ATPase in the plasma membranes by an angiotensin II-dependent mechanism. The abdominal aorta of anesthetized Sprague-Dawley rats was constricted with a ligature between the renal ...