A simple, precise, and accurate, isocratic, reversed RP-HPLC stability indicating method was developed and validated for determination of Nabumetone (Naphthyl acetic acid derivative). Isocratic RP-HPLC separation was achieved on Inert Sil ODS 3V (5μ, 25 cm× 4.6 mm, i.d.) column using mobile phase consisted of 0.47 g of tetrabutyl ammonium hydroxide sulphate (TBAH) dissolved in 1000 ml of a mixt...

The objective of the current study was to develop and validate a simple, precise and accurate isocratic stability-indicating reversed-phase high-performance liquid chromatography (RP-HPLC) assay method for the determination of spironolactone and furosemide in solid pharmaceutical dosage forms. Isocratic RP-HPLC separation was achieved on an SGE 150 × 4.6 mm SS Wakosil II 5C8RS 5-μm column using...

AIM Catechins profile, caffeine content and antioxidant activity of different green tea and white tea samples commercialized on the Romanian market were investigated. MATERIAL AND METHODS Five green tea samples and five white tea samples commonly available on the Romanian market were processed by infusion and the lyophilisates of infusions were analyzed. Total phenolic content was determined ...

RP-HPLC has largely been the analytical method of choice in the pharmaceutical industry for many years because of the poor aqueous solubility and hydrophobicity of most small molecule drug candidates. RP-HPLC coupled to MS has provided an excellent analytical tool for qualitatively and quantitatively determining levels of drug molecules or drug metabolites for studies such as purity assessment ...

Caseinomacropeptide (CMP) is a heterogeneous C-terminal fragment (residues 106 to 169) of bovine milk kappa-casein composed of glycosylated and phosphorylated forms of different genetic variants. We have demonstrated that CMP has growth-inhibitory activity against the oral opportunistic pathogens Streptococcus mutans and Porphyromonas gingivalis and against Escherichia coli. CMP was fractionate...

A simple, precise, rapid and accurate RPHPLC method was developed for the estimation of Pomalidomide (PML) in capsule dosage forms. An XTerra RP C18, (250 x 4.6 with 5 microns particle size) and the mobile phase, consisting of 0.03M KH2PO4 in water adjusting the pH-3.2 with O-Phosphoric Acid: Acetonitrile in ratio of 20:80 v/v & Acetonitrile HPLC Grade: Water HPLC Grade (50:50 v/v) was used as ...

a gradient reversed-phase high performance liquid chromatography (hplc) method was developed for the assay of cetrorelix acetate, a synthetic decapeptide with gonadotropin-releasing hormone (gnrh) antagonistic activity used in infertility treatment. the hplc method, which is used to determine cetrorelix in bulk and pharmaceutical dosage forms, was validated per ich guidelines. the chromatograph...

Aim: The current study dealt with the degradation behavior of Bilastine and kinetics a drug in solution state. Background: Very limited information on effect pH maximum stability has been published. In order to understand bilastine, aqueous studies were carried out, because such bilastine have not reported literature, further no methods about shelf-life determination bilastine. design involves ...

Validasi metode analisis adalah suatu proses dalam menjamin akurasi analisis, reprodusibel, spesifik dan stabil dari kisaran sesuai dengan tujuan penggunaan. mencakup semua prosedur yang menunjukkan pengukuran kuantitatif analit matriks biologis seperti plasma. Penentuan konsentrasi obat plasma merupakan hal kompleks karena kompleks. Metformin agen antihiperglikemik oral digunakan pengelolaan d...