Pyridoxamine-pyruvate aminotransferase is a PLP (pyridoxal 5'-phosphate) (a coenzyme form of vitamin B6)-independent aminotransferase which catalyses a reversible transamination reaction between pyridoxamine and pyruvate to form pyridoxal and L-alanine. The gene encoding the enzyme has been identified, cloned and overexpressed for the first time. The mlr6806 gene on the chromosome of a symbioti...

BACKGROUND Hepatic manifestations are one of the unusual manifestations of dengue infection. OBJECTIVES We conducted this study in order to study the pattern of serum aminotransferases and sequential changes before and after shock in Thai children with dengue infection. PATIENTS AND METHODS Children who were clinically and serologically diagnosed as dengue infection and were admitted to Kin...

Aspartate aminotransferase from Corynebacterium glutamicum (CgAspAT) is a PLP-dependent enzyme that catalyzes the production of L-aspartate and α-ketoglutarate from L-glutamate and oxaloacetate in L-lysine biosynthesis. In order to understand the molecular mechanism of CgAspAT and compare it with those of other aspartate aminotransferases (AspATs) from the aminotransferase class I, we determine...

We examined the effects of temperature on the activity and steady-state kinetics of aspartate aminotransferase (EC 2.6.1.1), using purified human soluble (s-AspAT) and mitochondrial (m-AspAT) isoenzymes, human serum, and porcine s-AspAT. All enzymes obeyed similar linear Arrhenius relationships over the range 20-40 degrees C. Apparent energies of activation (52.3 kJ.mol-1) and ratios of activit...

Aspartate aminotransferases (AspATs; EC 2.6.1.1) catalyze the conversion of aspartate and α-ketoglutarate into oxaloacetate and glutamate and are key enzymes in the nitrogen metabolism of all organisms. Recent findings suggest that the plasmodial enzyme [Plasmodium falciparum aspartate aminotransferase (PfAspAT)] may also play a pivotal role in energy metabolism and in the de novo biosynthesis ...

BACKGROUND Attention has long been drawn to the potentially harmful effects of coffee on health, however recent epidemiological studies have suggested unexpected, possibly beneficial effects of coffee against the occurrence of alcoholic liver cirrhosis and upon serum liver enzyme levels. METHODS We examined the potential inverse association between coffee drinking and serum concentrations of ...

This is a case report on a human immune deficiency virus (HIV) prevention trial participant who HIV seroconverted, developed hepatitis and rapidly progressed to acquired immunodeficiency syndrome (AIDS). A 24 year old HIV negative, non-pregnant participant consented to participate in the FEM-PrEP clinical trial. Her baseline parameters were normal; she was on oral contraception and was vaccinat...

Fipronil (FPN) is a broad-spectrum N-phenylpyrazole insecticide and has been used in agriculture and public health since the mid-1990s. The present study was designed to investigate the adverse effects of sub-chronic exposure to the FPN on the liver and kidney of male rats at three concentrations 0.1, 1 and 10 mg/L in drinking water for 45 days. Serum aspartate aminotransferases (AST), alanine ...

The present study was designed and conducted to fi nd the appropriate physical baseline values for hepatobiliary enzymes such as, alanine aminotransferases (ALT), aspartate aminotransferases (AST), alkaline phosphatase (ALP) and gamma glutamyltransferases (GGT) in adult healthy Murrah buffaloes in hot humid climatic conditions and also to assess the ideal storage condition for buffalo sera samp...