The nucleotide sequence of ribosomal 5.8 S RNA (also known as 7 S or 5.5 S rRNA) from Novikoff hepatoma ascites cells has been determined to be (see article). Estimations of the secondary structure based upon maximized base pairing and the fragments of partial ribonuclease digestion indicate that there may be five base-paired regions in the molecule, three forming a folding of the termini and t...

A comparative study revealed that Ehrlich ascites carcinoma (EAC) cells use glutamine plus inosine for regeneration of adenylates via the purine nucleotide cycle, whereas AS 30D hepatoma cells use adenosine instead. This observation can be correlated with the very low production of aspartate from glutamine in hepatoma cells. Although glucose is an important energy fuel for EAC, it cannot mainta...

Received for publication January 22, 1965. were fixed after injection were determined by the use of †̃25I-labeledantibody and radioautography and were found to be mainly in connective tissues and occasionally on sinusoid walls (5). The inicrosome fraction of cells is derived from the endoplasmic reticulum, which has been claimed to be contiguous to cell surface. Moreover, Wallach and Hager hav...

The guanine insertion enzyme from Escherichia coli catalyzes exchange of guanine located at the first position of the anticodon of tRNA with radioactive guanine (N. Okada and S. Nishimura, unpublished data). tRNA isolated from various tumors, including slowly growing Morris hepatoma 7794A, incorporated considerable guanine with E. coli guanine insertion enzyme, whereas tRNA isolated from all no...

SUMMARY It has been demonstrated that solid hepatoma lysosomes lack hyaluronidase activity, whereas normal rat liver lyso somes contain a high level of this enzyme. This result was also found with lysosomes from an ascites hepatoma, Walker carcinoma 256, and virally transformed BHK cells. In contrast, 0-yV-acetylglucosaminidase activity was shown to be present in lysosomes from tumor cells, alt...

It has been demonstrated that solid hepatoma lysosomes lack hyaluronidase activity, whereas normal rat liver lyso somes contain a high level of this enzyme. This result was also found with lysosomes from an ascites hepatoma, Walker carcinoma 256, and virally transformed BHK cells. In contrast, 0-yV-acetylglucosaminidase activity was shown to be present in lysosomes from tumor cells, although it...

We have previously demonstrated in a rat ascites hepatoma cell line (Yoshida AH 130) the presence of a glucose-activatable and amiloride sensitive Na+/H+ exchange (Cell Biol. Int. Rep., 1984, 8, 297-307). Amiloride is known to inhibit this exchange and to cause a cytoplasmic acidification, with inhibition of protein and DNA synthesis, in cells induced to grow. Amiloride appears also to penetrat...

Nucleolar antigens of normal rat liver, regenerating liver, and Novikoff ascites hepatoma cells were transplanted in vitro from polyadenylic acid-containing messenger RNAs isolated from the respective tissues and immunoprecipitated with specific antinucleolar antibodies and Protein A. By two-dimensional gel electrophoresis of the translation products, five antigens were detected in normal rat l...

from several normal and cancerous tissues were studied by means of the newly developed reverse-phase chromatography system, RP-5. Tyrosyl transfer RNA from normal rat, mouse, and hamster livers emerged as a single peak. All three species exhibited identical profiles. Tyrosyl transfer RNA from Escherichia coli required a considerably higher NaCl concentra tion for elution from the RP-5 column. T...

Accumulation of Ca2+ (+ phosphate) by respiring mitochondria from Ehrlich ascites or AS30-D hepatoma tumor cells inhibits subsequent phosphorylating respiration in response to ADP. The respiratory chain is still functional since a proton-conducting uncoupler produces a normal stimulation of electron transport. The inhibition of phosphorylating respiration is caused by intramitochondrial Ca2+ (+...