We show by confocal immunofluorescence microscopy that the water channel protein aquaporin-1, not previously identified within cardiomyocytes, localizes at 20 and 37°C to rat cardiomyocyte sarcolemmal caveolar membrane and subsarcolemmal cytoplasm of primary atrial myocyte cultures, dissociated atrial and ventricular myocytes, and in situ cardiomyocytes of atrial and ventricular frozen sections...

Tolvaptan, a vasopressin type-2 receptor antagonist, is utilized to ameliorate fluid retention following cardiac surgery. However, the optimal timing of tolvaptan administration considering novel biomarkers remains unknown. We prospectively included patients who underwent surgery between 2016 and 2020. measured perioperative trends free water reabsorption mediators including plasma arginine uri...

BACKGROUND Glial cells such as retinal Müller glial cells are involved in potassium ion and water homeostasis of the neural tissue. In these cells, inwardly rectifying potassium (Kir) channels and aquaporin-4 water channels play an important role in the process of spatial potassium buffering and water drainage. Moreover, Kir4.1 channels are involved in the maintenance of the negative Müller cel...

Water transport across the mammalian collecting tubule is regulated by vasopressin-dependent aquaporin-2 insertion into and retrieval from the apical cell membrane. To establish a cell line that properly expresses aquaporin-2 and its hormone-dependent shuttling, Madin-Darby canine kidney cells were stably transfected with an aquaporin-2 expression construct. Cells of a representative clone (wil...

The purpose of this study was to compare the expression of BSC-1 (bumetanide-sensitive Na+-K+-2Cl- cotransporter) in kidneys of spontaneously hypertensive rats (SHR) versus Wistar-Kyoto (WKY) rats by immunoblotting and reverse transcription-polymerase chain reaction. To determine the specificity of any observed changes in BSC-1 expression, we also compared expression of the thiazide sensitive N...

this paper investigates the mechanism of water transportation through aquaporin-4(aqp4) of ratbrain cells by means of molecular dynamics simulation with charmm software. the aqp4 wasembedded into a bilayer made of dimystroilphosphatylcholine (dmpc). the results illustrate thatwater molecules move through aqp4's channel with change of orientation of oxygen of eachwater molecule.

Wiederkehrende Episoden entzündlicher Schädigungen des Sehnervs und Rückenmarks sind als eigenständige Erkrankung schon aus historischen Überlieferungen bekannt. Im Jahr 2004 gelang die Identifikation pathognomonischen, gegen Aquaporin‑4 auf Astrozyten gerichteten Antikörpers. Zugehörige klinische Manifestationen werden Neuromyelitis-optica-Spektrum-Erkrankungen („neuromyelitis optica spectrum ...