Methods: Over a 6 month period, 33 patients with severe sepsis or septic shock were studied in the intensive care unit. Annexin V binding by leukocytes was determined daily using flow cytometry and FITC-labeled Annexin V. Transient leukocytosis, or peaks in leukocyte counts, were defined as individual increase of at least 30% within 2 days, followed by a decrease of at least 30% within the foll...

guish between apoptotic and necrotic cells, we performed an Annexin V assay with propidium iodide exclusion. In accordance with our TUNEL assay, .95% of the cells were Annexin V positive (unpublished results). Less than 5% of these Annexin V-positive cells showed simultaneous incorporation of propidium iodide. We therefore conclude that .95% of the plasmaderived cells are indeed in apoptosis an...

BACKGROUND There is evidence that circulating microparticles (MPs) and annexin (+) platelet-derived MPs (PDMPs) are increased in inflammatory bowel disease (IBD). The aim of our study was to characterize the abundance, origin, and annexin V binding of MPs in patients with IBD and correlate them with the disease characteristics. METHODS Case-control study of 46 IBD patients (23 Crohn's disease...

The Ca(2+)- and phospholipid-binding protein, annexin V, has been shown by an immune assay to represent 0.4% of total cell protein in cultured chick-embryo fibroblasts. Immunofluorescent localization studies indicate that in primary cultures the protein is abundant in the cytoplasm of the cells and also extends into the nucleus. Nuclear staining is no longer detectable, however, in approx. 25% ...

The ability of annexins, particularly annexin 1 (lipocortin 1), to inhibit phospholipase A2 (PLA2) is well known and a substrate depletion mechanism is now widely accepted as the explanation for most inhibitory studies. However, there are only a very limited number of reported studies involving annexins and the high-molecular-mass cytosolic PLA2 (cPLA2). In this study we have examined the effec...

Methods Tc-HYNIC-annexin V was prepared and its binding to apoptotic cell models of Parkinson’s disease was studied in vitro. Cellular models of Parkinson’s disease were produced by administering different concentrations of 1-methyl-4-phenylpyridinium to PC12 and SH-SY5Y cell lines. Cell apoptosis rates were analysed by flow cytometry. Annexin V was labelled with Tc by hydrazinonicotinamide (HY...

Biomineralization is a highly regulated process that plays a major role during the development of skeletal tissues. Despite its obvious importance, little is known about its regulation. Previously, it has been demonstrated that retinoic acid (RA) stimulates terminal differentiation and mineralization of growth plate chondrocytes (Iwamoto, M., I.M. Shapiro, K. Yagumi, A.L. Boskey, P.S. Leboy, S....