Abacavir sulphate was subjected to forced degradation under the conditions of hydrolysis (acid, alkali and neutral), oxidation, photolysis and thermal stress as prescribed by ICH. Eight degradation products were formed and their separation was accomplished on Waters XTerra C₁₈ (250 mm x 4.6 mm, 5 μm) column using 20 mM ammonium acetate:acetonitrile as a mobile phase in gradient elution mode by ...

Synthesis of the tripeptide Z-Phe-Arg-SerNH2 has been accomplished by a recombinant cysteine protease, cathepsin L1 from liver fluke (Fasciola hepatica), using Z-Phe-Arg-OMe as acyl acceptor and SerNH2 as nucleophile in 0.1 M ammonium acetate pH 9.0-12.5% v/v acetonitrile at 37 degrees C. LC-MS detection indicated tripeptide formation after 10 min, continuing up to 5.5 h. The ester Z-Phe-Arg-OM...

Catalytic activities of eight sulfonic acid group functionalized ionic liquids in water were compared for the hydrolysis of Sigmacell cellulose (DP ~ 450) in the 150-180°C temperature range by measuring total reducing sugar (TRS) and glucose produced. The catalytic activity of acidic ionic liquids with different cation types decreases in the order imidazolium > pyridinium > triethanol ammonium ...

This paper reports a development of microfluidic devices integrating an ion selective-field effect transistor (ISFET) sensor with a tantalum pentoxide as a sensing surface to detect protons. To attain high sensitivity in flow injection analysis, a novel on-line preconcentration of ionic analytes was demonstrated by integrating a pair of metal electrodes on a microchannel. In our proposed method...

Poly(7-glutamic a id) (PGA) production ill Bacillas suhtitis IF03335 was studied. When citric acid as a carbon source was added to a glutamic acid medium containing L-glutamic acid and ammonium sulfate, a large amount of pure PGA wEs produced. On the other hand, when glucose was added to the glutamic acid medium, a by-preduct was produced, which seemed to be a polysaccharide. Moreoyer, the mode...

I propose a single, quick method for measuring ammonia in urine and urea in plasma and urine. An ammonia-selective electrodie is used, set up on a microcell, which allows use of small sample volumes. Ammonia is measured directly after partial conversion of ammonium ions to NH3. Urea is measured after its hydrolysis by urease. With urine, the two procedures can be carried out successively in the...

Trypsin was purified from pyloric ceca of the starfish Asterina Pectinifera by ammonium sulfate precipitation, gel filtration, and cation-exchange chromatography. Final enzyme preparation was nearly homogeneous in sodium dodecyl sulfate-polyacrylamide gel electrophoresis and its molecular weight was estimated as approximately 28000. Optimum pH and temperature of A. pectinifera trypsin for hydro...

Proton balance is analysed in relation to the anaerobic and aerobic metabolism of carbohydrates, carbonic acids, amino acids and fat by considering oxidation, carboxylation, decarboxylation and phosphorylation reactions, as well as the influence of ammonium, on the acid-base status of animal tissues. The functional role of the adenylates, phosphagens and inorganic phosphate in acid-base balance...

L-asparaginase (ASNase) is an aminohydrolase enzyme widely used in the pharmaceutical and food industries. Although currently its main applications are focused on treatment of lymphoproliferative disorders such as acute lymphoblastic leukemia (ALL) acrylamide reduction starch-rich foods cooked at temperatures above 100 °C, use a biosensor detection monitoring L-asparagine levels high relevance....

Soybean trypsin inhibitor (STI) was obtained from simulated soybean whey wastewater through a sustainable method consisting of isoelectric precipitation, ammonium sulfate salting out, and gel filtration chromatography, the effect temperature, pH, pepsin on stability STI also discussed. The results showed that recovery rate inhibitory activity 89.47%, purity specific were 71.11%, 1442.5 TIU/mg i...