Objective(s): Production of a recombinant and immunogenic antigen using dengue virus type-3 envelope protein is a key point in dengue vaccine development and diagnostic researches. The goals of this study were providing a recombinant protein from dengue virus type-3 envelope protein and evaluation of its immunogenicity in mice. Materials and Methods: Multiple amino acid sequences of different i...

Nowadays, the clustering of proteins and enzymes in particular, are one of the most popular topics in bioinformatics. Increasing number of chitinase genes from different organisms and their sequences have beenidentified. So far, various mathematical algorithms for the clustering of chitinase genes have been used butmost of them seem to be confusing and sometimes insufficient. In the...

Sequence alignments and sequence similarity scores derived from them are the most common tools for comparing amino acid and DNA sequences. Different scoring schemes, from simple +6/-1 to PAM and BLOSUM scoring matrices have been devised for highlighting particular biological or evolutionary properties of the sequences to compare. However, most methods of classical, as well as of non-parametrica...

The nucleotide sequences of two DNA segments from Pseudomonas sp. strain CBS3 that code for two different haloalkanoic acid halidohydrolases were determined. Two open reading frames with coding capacities of 227 amino acids (corresponding to a molecular mass of 25,401 Da) and 229 amino acids (corresponding to a molecular mass of 25,683 Da) were identified as structural genes of 2-haloalkanoic a...

Lys 10 10 8 4 8 7 5 12 9 13 9 Try 4 4 4 1 2 1 6 3 4 3 4 6 His 0 0 0 1 0 3 I 4 1 0 0 Arg 2 2 2 6 3 3 4 4 8 6 2 Asp 18 17 16 20 17 1 3 1 4 22 28 25 21 28 Thr 4 5 8 6 9 6 8 14 16 8 8 Ser 14 8 7 7 8 4 4 11 11 15 12 Glu 19 21 18 17 18 1 6 1 7 25 22 16 23 26 Pro 4 4 5 4 6 3 4 5 7 5 3 Gly 17 14 14 19 14 11 19 25 28 21 19 Ala 14 6 18 18 15 11 15 24 20 15 12 CvS 1 3 2 5 5 3 2 5 2 1 1 Val 15 10 13 10 16 ...

A statistical method was developed for reconstructing the nucleotide or amino acid sequences of extinct ancestors, given the phylogeny and sequences of the extant species. A model of nucleotide or amino acid substitution was employed to analyze data of the present-day sequences, and maximum likelihood estimates of parameters such as branch lengths were used to compare the posterior probabilitie...

What kinds of amino acid sequences could possibly be protein sequences? From all existing databases that we can find, known proteins are only a small fraction of all possible combinations of amino acids. Beginning with Sanger's first detailed determination of a protein sequence in 1952, previous studies have focused on describing the structure of existing protein sequences in order to construct...

Manymulti-domain proteins have repetitive three-dimensional structures but nearly-random amino acid sequences. In the present paper, by using amodified recurrence plot proposed by us previously, we show that these amino acid sequences have hidden repetitions in fact. These results indicate that the repetitive domain structures are encoded by the repetitive sequences. This also gives a method to...