When 10 strains of lactic acid bacteria were incubated with 5'-hydroxyaverantin (HAVN), a precursor of aflatoxins, seven of them converted HAVN to averufin; the same reaction is found in aflatoxin biosynthesis of aflatoxigenic fungi. These bacteria had a dehydrogenase that catalyzed the reaction from HAVN to 5'-oxoaverantin (OAVN), which was so unstable that it was easily converted to averufin....

an experiment was conducted to reevaluate the digestible lysine requirement of arian male broilers by comparing the performance of chicks fed different levels of cottonseed meal (csm) on a total amino acid (aa) versus a digestible aa basis. four hundred and thirty two (432) arian male broiler chicks were allotted to 18 treatments with four repli-cates of six chicks each in a completely randomiz...

In this study, we report cDNA sequences of the cytosolic NADP-dependent isocitrate dehydrogenase for humans, mice, and two species of voles (Microtus mexicanus and Microtus ochrogaster). Inferred amino acid sequences from these taxa display a high level of amino acid sequence conservation, comparable to that of myosin beta heavy chain, and share known structural features. A Caenorhabditis elega...

CsgA is a cell surface protein that plays an essential role in tactile responses during Myxococcus xanthus fruiting body formation by producing the morphogenic C-signal. The primary amino acid sequence of CsgA exhibits homology with members of the short-chain alcohol dehydrogenase (SCAD) family and several lines of evidence suggest that NAD(P)+ binding is essential for biological activity. Firs...

Several different experimental approaches have shown the presence of tryptophan in malate dehydrogenase from pig heart mitochondria. The methods used were (a) fluorescence measurement of tryptophan content after acid hydrolysis and separation of amino acids by paper chromatography, (b) recovery of tryptophan labeled with tritium after incubation of the enzyme with tritiated substrates, (c) dete...

The effect of cobalt on the lactic, pyuruvic acid contents and activity levels of aldolase, lactate dehydrogenase (LDH), succinate dehydrogenase (SDH) and isocitrate dehydrogenase (ICDH), glucose-6-phosphate dehydrogenase (G6-PDH) and glutamate dehydrogenase ( GDH) were studied. The decrease in the lactic acid content and increase in the pyruvic acid content in the silk gland of experimental si...

drogenase in normal human skin. In comparison with glutamate dehydrogenase, which catalyzes reversible oxidative deamination, the transaminases catalyze transfer of amino groups, usually from an amino acid to a ketoacid, without net loss of nitrogen. In other words, transamination represents the intermolecular exchange of amino nitrogen. Transamination plays a significant role in metabolism by ...

Dihydrolipoamide dehydrogenase is a common component of mammalian multienzyme complexes that decarboxylate alpha-ketoacids and catabolize glycine. The common function is to reoxidize a reduced lipoate component of each complex, thereby preparing that lipoate for another round of catalysis. Regions within dihydrolipoamide dehydrogenase involved in association with other proteins of the complexes...

Treatment of dogfish l& lactic dehydrogenase with carboxypeptidase A releases 1 mole of phenylalanine per 36,000 g of the enzyme and no other amino acids. A mixture of carboxypeptidases A and B releases 1 mole of phenylalanine and 1 mole of lysine per 36,000 g of the lactic dehydrogenase. The amino terminus of dogfish M4 lactic dehydrogenase does not react with either dansyl chloride or the Edm...

The nucleotides 8-amino-, 8-methylamino-, and 8-dimethylaminoadenylic acid have been synthesized and their preferred conformations about the glycosyl bond in qaueous solution have been determined by 1H nuclear magnetic resonance spectroscopy. Paramagnetic relaxation studies, nuclear Overhauser enhancement measurements, chemical shifts, and coupling constant comparisons indicate that their is ro...