BACKGROUND The aim of this study was to explore the mechanism by which amentoflavone (AME) improves insulin resistance in a human hepatocellular liver carcinoma cell line (HepG2). METHODS A model of insulin resistant cells was established in HepG2 by treatment with high glucose and insulin. The glucose oxidase method was used to detect the glucose consumption in each group. To determine the m...

Seven flavonoid compounds isolated from the EtOAc extract of Garcinia mackeaniana leaves include 2”,6”-di-O-acetylvitexin (1), 2”-O-acetylvitexin (2), vitexin (3), amentoflavone (4), apigenin (5), kaempferol (6), and quercetin (7) were evaluated for biological activities. Results showed that both (6) exhibited strong antioxidant activity with IC50 values 9.5±0.38 7.4±0.30 μg/ml, respectively. C...

In the present study, models were developed to estimate the altitudinal variation in secondary metabolite contents of Hypericum orientale growing wild in “Güldağı” mountain, Turkey. Aerial parts of flowering 30 individuals were collected at six different altitudes (500, 1150, 1650, 2100, 2720 and 3250 m). Actual secondary metabolite contents of plants were measured by High Performance Liquid Ch...

Seasonal variations of the phytochemicals contents in needles of T. wallichiana var. mairei due to the effects of growth meteorological parameters were investigated in this study. The needles of T. wallichiana var. mairei were collected from different months and the contents of taxoids (paclitaxel, 10-deacetylbaccatin III (10-DAB), baccatin III, cephalomannine, 10-deacetyltaxol (10-DAT)), flavo...