نتایج جستجو برای: amelogenin
تعداد نتایج: 619 فیلتر نتایج به سال:
Two features make the tooth an excellent model in the study of evolutionary innovations: the relative simplicity of its structure and the fact that the major tooth-forming genes have been identified in eutherian mammals. To understand the nature of the innovation at the molecular level, it is necessary to identify the homologs of tooth-forming genes in other vertebrates. As a first step toward ...
The ability to determine the sex of an individual based on DNA evidence can be crucial in instances such as identification of victims of mass disaster, missing persons investigations, and sexual assault cases. The Y chromosome marker amelogenin is currently in widespread use for determination of chromosomal sex of an unknown DNA donor and differentiating the relative contributions of male and f...
In order to have a good perspective of wild animals, it is necessary to determine their population and genetic structure. It provides an opportunity to decide on better conservation managements. In the wilderness, due to the escapable nature and sometimes not having the distinguishable bisexual appearance, sex identification could be difficult by observing animals. The X- and Y- chromosome link...
Tooth enamel has the highest degree of biomineralization of all vertebrate hard tissues. During the secretory stage of enamel formation, ameloblasts deposit an extracellular matrix that is in direct contact with the ameloblast plasma membrane. Although it is known that integrins mediate cell-matrix adhesion and regulate cell signaling in most cell types, the receptors that regulate ameloblast a...
Although molecular dating of cladogenetic events is possible, no molecular method has been described to date the acquisition of various tissues. Taking into account the specificity of the major protein in enamel in formation (amelogenin), we were able to develop such a method for enamel. Indeed, because the amelogenin protein is exclusively involved in enamel formation and mineralization and be...
and Emdogain® to Plastic by Enzyme-linked Immunosorbent Assays (ELISA) To assess whether recombinant amelogenin and Emdogain® used in the cell attachment experiments indeed coupled to plate surfaces, we coated a two-fold serial concentration range (1.5 g–1.5 and 0 ng/well) of each protein in duplicate on Dyclone 2HB 96-microwell plates overnight at 4°C in MAM, imitating the conditions used in t...
In vitro studies on interactions between amelogenins and calcium phosphate crystals are critical for elucidating biomineralization mechanisms of tooth enamel. This work was aimed at investigating the effects of native porcine amelogenins on octacalcium phosphate (OCP) crystal growth in a gelatin gel. We prepared OCP mineral discs by circulating calcium and phosphate solutions on the opposite en...
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