During the past six or seven years, high resolution nuclear magnetic resonance (NMR) has been established as a powerful tool for the study of cellular metabolism in isolated cells and organs. The non-invasive nature of the measurement offers many advantages over conventional analytical techniques, but limitations on sensitivity restrict the method to the observation of molecules present in the ...

The recent success of the human genome project and the continued accomplishment in obtaining DNA sequences for a vast array of organisms is providing an unprecedented wealth of information. Nevertheless, an abundance of the proteome contains hypothetical proteins or proteins of unknown function, where high throughput approaches for genome-wide functional annotation (functional genomics) has evo...

The soluble proteins of a newborn human lens exhibit almost no non-tryptophan fluorescence. On aging, an increase in the fluorescence of all native crystallins is found except for 72-crystallin. With formation of nuclear cataract, a further increase is seen for 7,-crystallin. The fluorophore, excitation 355 nm/emission 420 nm, is mainly associated with one species of the Yi-crystallin populatio...

Active transport is critical for cellular organization and function, and impaired transport has been linked to diseases such as neuronal degeneration. Much long distance transport in cells uses opposite polarity molecular motors of the kinesin and dynein families to move cargos along microtubules. It is increasingly clear that many cargos are moved by both sets of motors, and frequently reverse...

Intracellular transport of membrane organelles occurs along microtubules (MTs) and actin filaments (AFs). Although transport along each type of the cytoskeletal tracks is well characterized, the switching between the two types of transport is poorly understood because it cannot be observed directly in living cells. To gain insight into the regulation of the switching of membrane organelles betw...

eighteen kilometers long coastal stretch from the new mangalore port in the north totalapadi in the south has been studied in order to understand shoreline changes and erosion/ accretion patterns that have taken place due to the natural processes and anthropogenic activities. shoreline changes and quantification of beach erosion/accretion were made using the survey of india topomap, multidated ...