The sea cucumber Holothuria leucospilota has high me-dicinal value and rich nutritional edible value, and thus is a commercially important aquatic product in China. Microsatellite loci were developed and screened using a fast isolation protocol and amplified fragment length polymorphism of sequences containing repeats. In this study, 16 novel polymorphic microsatellite markers in H. leucospilot...

UNLABELLED PREMISE OF THE STUDY Microsatellite markers were developed for Isodon longitubus to study the natural hybridization of the species and its congeners. • METHODS AND RESULTS A total of 10 primer sets were developed for I. longitubus. From the initial screening, all of 10 loci were polymorphic with five to 19 alleles per locus in the Mt. Ishizuchi population, whereas nine loci were...

Pedigree information and phenotypic records are the key features in animal genetics and breeding. Otherwise best methods for genetic evaluation and estimation of parameters will be tending to no correct results. This study was aimed to investigate and verify pedigree relations among 76 individuals who were genotyped for 3 fluorescent labeled microsatellite markers picked up from FAO-ISAG joint ...

mahi sefid (cyprinidae) is an economically important fish in the south caspian sea. specimens was collected from the lamir river, sefid rud, shir rud and tajan rivers and analyzed at 38 microsatellite dna loci. eight primers out of the thirty analyzed loci were polymorphic in all sampling regions. the highest observed heterozygosity (1) was in locus ca1 in sefid rud and lowest was 0.07 in locus...

This paper describes a single tube assay for 20 fluorescent labelled Y-STR loci, which is rapid and robust state-of-art multiplex system. Reaction conditions, including annealing temperature, concentration of primers, magnesium and template DNA, DNA polymerase and reaction volume, were optimised to yield robust amplification. The assay could withstand moderate fluctuations in reaction parameter...

A study of genic variation in natural population of D. melanogaster was undertaken (1) to obtain a better estimate of heterozygosity by sampling a relatively large number of gene loci and (2) to identify different groups of polymorphic loci whose variation patterns might suggest different kinds of selection forces. A total of 117 gene loci (coding for 79 enzymes and 38 abundant proteins) were s...

Twenty microsatellite DNA markers were developed for sea cucumber and used to investigate polymorphisms of 60 wild Apostichopus japonicus individuals collected from China. It revealed that all the markers were polymorphic. A total of 164 alleles were detected at 20 loci. The number of alleles per locus varied from 3 to 17 with an average of 8.2, and the expected heterozygosities of each locus r...

Twenty-four simple sequence repeat markers were developed for Phakopsora pachyrhizi, a fungal pathogen of soybean (Glycine max) and other legumes. All 24 of the loci were evaluated on 28 isolates of P. pachyrhizi. Twenty-one loci were polymorphic, with allelic diversity ranging from two to eight alleles, and null alleles were observed for eight of the 24 loci. A preliminary screen with the clos...

In the present study we evaluated the amount and distribution of genetic variation by using RAPD marker variation of 12 markers loci in three broodstock groups of rainbow trout. A total of 47 polymorphic bands were observed in Iranian strain, average number of bands was 10 and average number of polymorphic bands per primer was 3.92. The total detected bands in rainbow trout strain originated fr...