Introduction: One of the vital enzymes during spermatogenesis, which is one of the pluripotency factors of stem cells and contributes to maintaining their pluripotency is alkaline phosphatase. ZBTB16 and ZFP proteins are critical elements in stem cells which are expressed in pluripotent stem cells and maintain their pluripotency due to their role in messaging pathways. Material & Methods: The ...

conclusions our findings clearly indicate a synergistic effect of waterpipe smoke, immobilization and darkness stress on serum ck levels, whereas serum alp levels of experimental groups have shown no significant synergism for the mentioned factors. results the results indicated enhanced serum ck and alp levels in all groups compared with control (p < 0.05). also, there was a significant differe...

despite the availability of various alkaline phosphatase (alp) isoenzymes, the calf enzyme is being used in current enzyme assays as the detector enzyme. the glycosylation pattern of this enzyme makes it a suitable ligand for binding to wheat germ agglutinin lectin (wga). as a result of this property, the enzyme can not be used as a conjugate with this lectin, and the calf enzyme conjugates can...

introduction: the aim of this study was to evaluate the correlation between serum levels of ovarian and gonadotropin hormones, age and number of follicles with follicular alkaline and acid phosphatase activity in infertile women under controlled ovarian hyperstimulation. methods: after collection of follicular fluid and calculation of the number of follicles, the specific activity of alkaline (...

A monoclonal antibody, NP57, was produced and used against the neutrophil granule protein elastase, which selectively stain neutrophils in cryostat and paraffin wax sections. The antibody stains neutrophils and a subpopulation of monocytes in blood smears and neutrophil precursors in bone marrow smears, and gives positive reactions with the cell lines HL60 and U-937. It labelled the blast cells...

AIMS To examine the distribution of PAI-1 antigen in normal and cirrhotic liver and liver with metastases. METHODS Sections of normal and cirrhotic liver and liver with metastases were stained using the alkaline phosphatase antialkaline phosphatase (APAAP) technique and monoclonal antibody specific for plasminogen activator inhibitor (PAI-1). RESULTS PAI-1 antigen was identified as discrete...

BACKGROUND/AIM It has been demonstrated that lymphocytes, plasma cells, macrophages and neutrophil granulocytes represent the predominant cells of the inflammatory lesion of the dental granulomas. Other cells, such as mast cells, eosinophils, dendritic cells comprise minor, but functionally important cell populations. Most of the data considering cells that take part in these processes have bee...

There are a number of methods currently available for the quantitative estimation of acid and alkaline phosphatase activity in biologic materials (l-5). In all the techniques, a phosphoric acid ester serves as a substrate and calorimetric determinations are made either of the inorganic phosphate or of the organic moiety of the substrate released by enzymatic hydrolysis. A description of a new m...

Intestinal alkaline phosphatase activity was measured using levamisole inhibition, and results were compared with a previously reported method using L-phenylalanine. Sixty two per cent intestinal, 39% placental, and 1.3% of either bone or liver alkaline phosphatase activity remained when alkaline phosphatase activity was inhibited in a 2-amino-2-methyl-1-propanol (AMP) buffer reagent system wit...

A technique using alkaline phosphatase histochemistry on routine sections of four jejunal biopsy specimens and one necropsy sample was applied to show that alkaline phosphatase activity, normally present in the brush border, occurs in the enterocytes of patients with microvillus inclusion disease. Sections were cut at 5 micron, mounted on to glass slides, and dried overnight at 37 degrees C bef...