Plasma glutathione (GSH) concentration in rats increased from approximately 15 to 30 microM after administration of GSH either as a liquid bolus (30 mumol) or mixed (2.5-50 mg/g) in AIN-76 semisynthetic diet. GSH concentration was maximal at 90-120 min after GSH administration and remained high for over 3 h. Administration of the amino acid precursors of GSH had little or no effect on plasma GS...

The aim of this study was to investigate whether the preincubation of brain homogenates with L-phenylalanine (Phe), L-cysteine (Cys) or reduced glutathione (GSH) could reverse the free radical effects on Na+,K+-ATPase activity. Two well established systems were used for the production of free radicals: 1) FeSO4 (84 microM) plus ascorbic acid (400 microM) and 2) FeSO4, ascorbic acid and H2O2 (1 ...

umami (the fifth taste sensation)-component of tea and is contained 2—3% in tea leaves. Theanine is a glutamate derivative and is used extensively as supplements and beverages, because it has a brain nerve function such as a relaxing effect due to inducing alpha waves. But there are few reports about pharmacological action of theanine in comparison with catechin or caffeine, major tea component...

Alzheimer’s disease is a common neurodegenerative disease. Microglia induces oxidative stress in the brain for engulfing bacteria and viruses. The accumulating data indicate that apoptosis are two main actors induction of microglia activation-induced Disease. Oxidative one many triggers activate transient receptor potential melastatin 2 (TRPM2) channel. Glutathione (GSH) cytosolic antioxidant m...

This study was designed to investigate the oxidative stress parameters in laryngeal cancer and cancer-free adjacent tissues. Lipid peroxidation end product and the endogenous antioxidant components-CuZn superoxide dismutase (CuZn SOD) glutathione peroxidase (GSH Px), glutathione reductase (GSSG Rd) and glutathione (GSH)-were analysed by spectrophotometric and kinetic methods. Laryngeal cancer t...

Murine L1210 leukemia cells resistant to the antineoplastic agent L-phenylalanine mustard have a 1.52.0-fold elevation in their cellular GSH and GSSG content as compared to drug-sensitive cells. Cellular uptake of ~-[U-'~C]cystine and its incorporation into GSH of the resistant tumor are correspondingly elevated. Synthesis of y-glutamylcysteine, GSH, and GSSG is elevated 1.5-2.0-fold in cell-f...

This study examined age-related biochemical changes of the lung in an animal model of senile lung, senescence-accelerated mouse (SAM). Bronchoalveolar lavage (BAL) was performed on two strains of SAM, the senescence-prone strain (SAM P2) and the senescence-resistant strain (SAM R1), as well as on normal ageing C57 black mice (C57BL), aged 1-24 months. Elastase-like and elastase inhibitory activ...

Bisphenol A (BPA) has received considerable attention as an endocrine disrupting chemical and a possible substrate for genotoxic metabolites. BPA metabolism leads to formation of electrophilic o-quinones cable of binding to DNA and other endogenous nucleophiles. We have structurally identified the products resulting from the reaction of bisphenol A-3,4-quinone (BPAQ) with N-acetylcysteine (NAC)...

In the current research, edible meat of freshwater clam Corbicula fluminea was subjected to enzymatic peptide extraction using animal protease. The protein hydrolysate (CPH) contained 45.78% essential amino acids and 44.97% hydrophobic showed high alcohol dehydrogenase (ADH) activation in vitro. Four fractions were divided from CPH by ultrafiltration further investigated for their hepatoprotect...