Specimens from the Harderian gland, the lacrimal gland and bursa of Fabricius in ducks with chlamydiosis were processed by the classical histological methods and permanent preparations were obtained. On single and serial histological cross-sections from material fixed in non-aqueous fixator, histochemical tests for PAS reactivity, staining with alcian blue at pH 1.0 and 2.5 as well as staining ...

The histochemical localization of acid polysaccharides involves difficulties of fixation and usually reactions of uncertain specificity. Thus, the aqueous phase used in most fixatives is liable to elute at least part of the material, while most staining reactions lack specificity. The latter problem has lately been successfully attacked by the use of the salt-controlled alcian blue technique (S...

The microstructure of oesophagus and stomach in grey-backed shrike were examined by means of light microscopy. The results showed that the oesophagus glands were mucous glands, the shedding glandular cells and the mucin from the oesophagus glands secreted to the lumen. The number of the glands in the thoracic part was less than that of in the cervical part. The lumens of the glands showed an in...

Differentiation of mouse embryonic stem (ES) cells via embryoid bodies was established as a suitable model to study development in vitro. Here, we show that differentiation of ES cells in vitro into chondrocytes can be modulated by members of the transforming growth factor-beta family (TGF-beta(1), BMP-2 and -4). ES cell differentiation into chondrocytes was characterized by the appearance of A...

FIVE MUCOPOLYSACCHARIDE STAINS INHIBIT PLATELET AGGREGATION INDUCED BY SEVERAL AGGREGATING AGENTS: with two stains inhibition is competitive. Alcian blue, 0.05 mg/ml, added to platelet-rich plasma potentiates ADP-induced aggregation. Alcian blue, 0.5 mg/ml, itself produces platelet aggregation, probably causing the release reaction. Three of these stains produce aggregation of red cells. They a...