To evaluate phosphorus (P) availability and the role of microorganisms in P dynamics barley rhizosphere, we constructed a rhizobox using two arable Andosols under different fertilization management regimens cultivated (Hordeum vulgare L. cv. Minorimugi) for 5 weeks. The phosphatase-labile pool organic (Po) was assessed phosphatase-addition approach combination with chemical extraction Po from s...

Muscle extracts were subjected to fractionation with ethanol, chromatography on DEAE-cellulose, precipitation with (NH4)2SO4 and gel filtration on Sephadex G-200. These fractions were assayed for protein phosphatase activities by using the following seven phosphoprotein substrates: phosphorylase a, glycogen synthase b1, glycogen synthase b2, phosphorylase kinase (phosphorylated in either the al...

Many phosphorus release experiments have been conducted in the water level fluctuation zone (WLFZ). However, effects of rhizospheres on risk soil remain unclear. In this study, twenty-four samples were collected from WLFZ Pengxi River, largest tributary northern Three Gorges Reservoir. Physicochemical properties, forms and phosphatase activity rhizosphere near-rhizosphere soils investigated. Bo...

introduction: the present study was undertaken to produce alkaline phosphatase anti- alkaline phosphatase (apaap) complex and to use it in one of the most practical methods of determining the site of antigen in tissue or in cell, i.e. apaap technique, and its comparison with similar foreign products. materials and methods: in this basic and applied study the secreted antibodies of the two hybri...

Alkaline phosphatase from human first trimester placentas was purified, characterized, and compared with alkaline phosphatases from term placenta and liver. Three forms of first trimester placental alkaline phosphatase (I, IIa, and IIb) were isolated; their relative amounts were 35%, 39%, and 26%, respectively. Phosphatases I and IIa were found to be dimers, whereas phosphatase IIb appeared to ...

introduction: this study was performed to establish a complementary method to the diagnosis of complete hydatidiform mole and its differentiation from the other cases of gestational trophoblastic disease by measuring the total (alp) and placental alkaline phosphatase (plap) specific activity. materials and methods: serum and tissue extracts from 13 patients, 13 normal non-pregnant and 30 pregna...