Spindle pole bodies (SPBs) were isolated from the yeast Saccharomyces cerevisiae by an adaptation of the Kleinschmidt monolayer technique. Spheroplasts prepared from the cells were lysed on an air-water interface. Spread preparations were picked up on grids, transferred to experimental test solutions, and prepared for whole-mount electron microscopy. Using purified exogenous tubulin from porcin...

Sperm whale (Physeter macrocephalus) clicks have a multi-pulse structure, a result of the reflection of sound energy between air sacs in the spermaceti organ. Although previous research revealed that usual clicks (used for echolocation) recorded away from a vocalizing whale's longitudinal axis have waveforms with poorly defined pulse structures, it has been unknown whether sperm whale coda clic...

S. cerevisiae Ipl1, Drosophila Aurora, and the mammalian centrosomal protein IAK-1 define a new subfamily of serine/threonine kinases that regulate chromosome segregation and mitotic spindle dynamics. Mutations in ipl1 and aurora result in the generation of severely aneuploid cells and, in the case of aurora, monopolar spindles arising from a failure in centrosome separation. Here we show that ...

BACKGROUND The Aurora kinases control multiple aspects of mitosis, among them centrosome maturation, spindle assembly, chromosome segregation, and cytokinesis. Aurora activity is regulated in part by a subset of Aurora substrates that, once phosphorylated, can enhance Aurora kinase activity. Aurora A substrate activators include TPX2 and Ajuba, whereas the only known Aurora B substrate activato...

Centrosomes mature as cells enter mitosis, accumulating gamma-tubulin and other pericentriolar material (PCM) components. This occurs concomitant with an increase in the number of centrosomally organized microtubules (MTs). Here, we use RNA-mediated interference (RNAi) to examine the role of the aurora-A kinase, AIR-1, during centrosome maturation in Caenorhabditis elegans. In air-1(RNAi) embry...

malignant transformation of ameloblastomas arising from an odontogenic cyst or de novo is wellrecognized. malignancies in ameloblastomas may involve metastasis or a local dysplastic change in the tissue. the latter are classified as ameloblastic carcinomas. a 75-year-old male presented with a mandibular cystic swelling, with no evidence of metastasis. dysplastic ameloblastic cells with spindlec...

Although many countries banned of its usage, carbofuran (CF) is still one of the most commonly used carbamate derivative insecticides against insects and nematodes in agriculture and household, threatening the human and animal health by contaminating air, water, and food. Our goal was to evaluate the potential toxic effects of CF on mammalian oocytes besides mitotic cells. Caspase-dependent apo...

Intelligent knowledge based system (IKBS) is developed for optimizing dry CNC turning process using Taguchi method, CNC Machine, EN19 steel as the work piece material, andCutting Insert. Tool wear and spindle loading which are the machining parameters, spindle speed, feed rate, and depth of cut, areoptimized through the intelligent knowledge based system (IKBS). The experimental CNC turning mac...

Axial thermal deformation and axial stiffness are the leading technical indicators affecting face-grinding spindle manufacturing accuracy. However, supported by rolling bearings lubricated with grease has a disadvantage regarding temperature rise, stiffness, rotational To overcome this issue, study proposes novel combined bearings, which include water-lubricated hydrostatic thrust bearing two a...