Sixty isolates of Yersinia enterocolitica from pigs belonging to 1A and 4 biotypes, and one non-typeable isolate were investigated in order to determine the occurrence of genes directly connected with pathogenicity of Y. enterocolitica by using a multiplex PCR. The multiplex PCR assay was based on the amplification of the ail, ystA, ystB, and yadA genes in one reaction. The bacterial adhesion t...

Yep-phi is a T7-related bacteriophage specific to Yersinia pestis, and it is routinely used in the identification of Y. pestis in China. Yep-phi infects Y. pestis grown at both 20°C and 37°C. It is inactive in other Yersinia species irrespective of the growth temperature. Based on phage adsorption, phage plaque formation, affinity chromatography, and Western blot assays, the outer membrane prot...

Abstract Background Identifying lignocellulose recalcitrant factors and exploring their genetic properties are essential for enhanced biomass enzymatic saccharification in bioenergy crops. Despite modification of major wall polymers has been implemented reduced recalcitrance engineered crops, it could most cause a penalty plant growth yield. Alternatively, is increasingly considered to improve ...

During the development of B and T cells, a number of genes undergo rearrangement. In this paper we have studied the structure of the T cell gamma-chain gene in primary lymphomas and cell lines derived from patients with lymphoma. Samples derived from patients with angioimmunoblastic lymphadenopathy (AIL), Lennert's lymphoma, and Hodgkin's disease were also examined. TcR gamma was rearranged in ...

f3 real. (See, for instance, [1], p. 224, ex. 4.) We offer the following proof whic h, although it also uses Bieberbach's result, is considerably different. PROOF: Let cP (z) J~ . Then cP sends the open unit disk into itself. Let cP 1= cP and inductively 1 a? define cPn=cP OcPlI t. If cPll(Z)=An,1 z+AIl,z Z2+ . .. ,it is clear that A"'=M' A I ,2= M' AII ,I = A", AIl I,t, and A II ,2 = AI ,I A n...

In this report, we describe the development and evaluation of a 5' nuclease PCR assay for the detection of pathogenic Yersinia enterocolitica (YE). The assay targets the chromosomally encoded invasion gene ail. Three different primer/probe sets (TM1, TM2, and TM3) amplifying different, yet overlapping, regions of ail were examined for their specificity and sensitivity. The TM1 set displayed the...

In the present study, we used newly developed F(11) generation mouse advanced intercross lines (AIL) to fine map Pas1-3 quantitative trait loci (QTL). The (A/J x C57BL/6) F(11) AIL mouse population was created by crossing lung tumor-resistant C57BL/6 mice with lung tumor-susceptible A/J mice. By selectively genotyping 30% of the population, we have confirmed the Pas1 QTL and narrowed it to an i...

Let U(n, T) be the set of ail matrices of O's and l's of order n with exactly r 0's. We obtain an upper bound for the permanent of a matrix in U(n, t). For 0 <r < 2n and for nz-2n C r < n2-n we determine all matrices in U(n, r) with maximum permanent.