In both applied and basic research, Agrobacterium-mediated transformation is commonly used to introduce genes into plants. We investigated the effect of three Agrobacterium tumefaciens strains and five transferred (T)-DNA origins of replication on transformation frequency, transgene copy number, and the frequency of integration of non-T-DNA portions of the T-DNA-containing vector (backbone) int...

Beardsley, Robert E. (Manhattan College, Bronx, N.Y.), and Jacques Lipetz Effects of 5-fluorouracil and 5-fluorodeoxyuridine on growth and tumor-inducing ability of Agrobacterium tumefaciens. J. Bacteriol. 92:346-348. 1966.-Agrobacterium tumefaciens B6, grown in the presence of 5-fluorouracil or 5-fluodeoxyuridine, exhibited a prolonged lag phase. The tumor-inducing ability of bacteria grown in...

Umbilicaria muehlenbergii strain KoLRILF000956 is amenable to Agrobacterium tumefaciens-mediated transformation (ATMT), making it the only known genetically tractable lichen-forming fungus to date. We report another advancement in lichen genetics, a draft genome assembly for U. muehlenbergii with a size of 34,812,353 bp and a GC content of 47.12%, consisting of seven scaffolds.

Influence of different Agrobacterium rhizogenes strains on hairy roots induction and secondary metabolites production in Datura innoxia Mill.

Methods for the transformation of barley using Agrobacterium-mediated techniques have been available for the past 10 years. Agrobacterium offers a number of advantages over biolistic-mediated techniques in terms of efficiency and the quality of the transformed plants produced. This chapter describes a simple system for the transformation of barley based on the infection of immature embryos with...

Members of the genus Agrobacterium cause the neoplastic diseases crown gall, hairy root, and cane gall on numerous plant species. Extensive genetic analyses conducted in the 1980s identified key bacterial genes involved in virulence. During the past decade, however, genomic technologies have revealed numerous additional bacterial genes that more subtly influence transformation. The results of t...

Genetic transformation mediated by Agrobacterium involves the transfer of a DNA molecule (T-DNA) from the bacterium to the eukaryotic host cell, and its integration into the host genome. Whereas extensive work has revealed the biological mechanisms governing the production, Agrobacterium-to-plant cell transport and nuclear import of the Agrobacterium T-DNA, the integration step remains largely ...

Agrobacterium-mediated transformation is a major technique for the genetic engineering of plants. However, there are many economically important crop and tree species that remain highly recalcitrant to Agrobacterium infection. Although attempts have been made to "improve" transformation by altering the bacterium, future successes might come from manipulation of the plant. Recent studies that id...

An efficient Agrobacterium-mediate transformation method was developed by employing different duration of sonication, Agrobacterium strains, type of inoculation medium and concentrations of acetosyringone in Arya cultivar of wheat. Immature embryos were used as an explant for inoculation with Agrobacterium tumefaciens harboring the recombinant pBI121 plasmid. Among the durations of sonication t...

A critical step in the development of Agrobacterium tumefaciens-mediated transformation in orchid genus is the establishment of optimal conditions for T-DNA gene delivery into protocorm-like bodies from which the whole plantlets can be regenerated. As a first step in the development of a successful Agrobacterium tumefaciens-mediated transformation for Dendrobium savin white, factors influencing...