AIM The objective of the current study is to determine the concentration of aflatoxin B1 using high-performance liquid chromatography (HPLC) with a photodiode array (PDA) detector. MATERIALS AND METHODS Aflatoxin B1 certified reference grade from Trilogy Analytical Laboratory dissolved acetonitrile (ACN) at 10 µg/mL was using standard assessment. HPLC instruments such as ultraviolet-PDA detec...

The possibility of a low dose threshold for rat liver macromolecular binding of aflatoxin B1 was investigated. Doses in ng/kg of radiolabeled aflatoxin B1 produced measurable covalent binding of aflatoxin to DNA, RNA, and protein, and the extent of this binding increased linearly over a dose range of 10 to 1000 ng/kg. Macromolecular adduct formation was observed at the lowest dose used (10 ng/k...

A high-affinity IgM monoclonal antibody specific for aflatoxins was covalently bound to Sepharose 4B and used as a preparative column to isolate aflatoxin derivatives from the urine of people and experimental animals who had been exposed to the carcinogen environmentally or under laboratory conditions. Aflatoxin levels were quantified by radioimmunoassay and high-performance liquid chromatograp...

Aflatoxin M1 is the foremost metabolite of aflatoxin B1 in humans and animals, which may be present in animal products from animals fed with aflatoxin B1 contaminated feed. In this study a high performance liquid chromatography method for determination of aflatoxin M1 in eggs was described. The egg samples were diluted with warmed water and the toxin was immunoextracted followed by fluorescence...

aflatoxin b1 (afb1) is a secondary metabolite produced by some aspergillus species, which haveharmful impacts on human health such as carcinogenicity, teratogenicity, acute and chronic toxicity.in this study, the occurrence of afb1 in pistachio nuts during various preparing processes wasinvestigated in two pistachio farms from qazvin province, iran, from september to november 2012.high performa...

Background: This cross sectional study was conducted to evaluate the effect of &gamma-rays and electron-beam irradiation with a commercial toxin binder-milbond-TX on the performance, feed components, and meat quality of Japanese quails challenged with aflatoxin B1. Methods: Overall, 168 One-d-old chicks (Japanese quails) were allocated to eight treatments with three replicates based on a com...

background: aflatoxins cause health hazards to human and animals and has also economical problems. therefore, the de­toxification effect of citric acid was investigated in rice as the main food of iranian people. methods: initially 275 samples of rice were examined for aflatoxins by hplc. the aflatoxins contaminated samples were later treated by aqueous citric acid and detoxification of aflatox...

Evaluation of kinetic model aflatoxin B1 adsorption by Saccharomyces cerevisiae treated with acid and ultrasonic in Sangak bread dough

West African Aspergillus flavus S isolates differed from North American isolates. Both produced aflatoxin B1. However, 40 and 100% of West African isolates also produced aflatoxin G1 in NH4 medium and urea medium, respectively. No North American S strain isolate produced aflatoxin G1. This geographical and physiological divergence may influence aflatoxin management.