NADH oxidase-overproducing Lactococcus lactis strains were constructed by cloning the Streptococcus mutans nox-2 gene, which encodes the H2O-forming NADH oxidase, on the plasmid vector pNZ8020 under the control of the L. lactis nisA promoter. This engineered system allowed a nisin-controlled 150-fold overproduction of NADH oxidase at pH 7.0, resulting in decreased NADH/NAD ratios under aerobic ...

Three Trichomonas vaginalis isolates with low in vivo susceptibilities to metronidazole (95% curative dose, greater than 3 X 100 mg kg-1 in subcutaneous infections in mice) were compared with strain ATCC 30001 and with four isolates exhibiting high in vivo susceptibilities (95% curative dose, less than 3 X 15 mg kg-1). Activity of pyruvate:ferredoxin oxidoreductase, anaerobic fermentation, and ...

The changes in yields and nutritive composition of whole crop wheat (Triticum aestivum L.) during maturation and effects of maturity stage and lactic acid bacteria (LAB) inoculants on the fermentation quality and aerobic stability were investigated under laboratory conditions. Whole crop wheat harvested at three maturation stages: flowering stage, milk stage and dough stage. Two strains of LAB ...

OBJECTIVE The objective of this study was to evaluate the effect of sodium diacetate (SDA) on fermentation profile, chemical composition and aerobic stability of alfalfa (Medicago sativa L.) silage. METHODS Fresh alfalfa was ensiled with various concentrations of SDA (0, 3, 5, 7, and 9 g/kg of fresh forage). After 60 days of the ensiling, the samples were collected to examine the fermentative...

Pichia guilliermondii is a Crabtree-negative yeast that does not normally exhibit respirofermentative metabolism under aerobic conditions, and methods to trigger this metabolism may have applications for physiological study and industrial applications. In the present study, CAT8, which encodes a putative global transcriptional activator, was disrupted in P. guilliermondii. This yeast's ethanol ...

For many years bacteriologists have observed that some bacteria produce acid from carbohydrates only under aerobic conditions while others produce acid both under aerobic and anaerobic conditions. The significance of these observations does not seem to have been appreciated generally by taxonomists. Studies of bacterial physiology have made it increasingly evident that the bacterial metabolism ...

The fiddler crab, Uca pugilator, used sideways octapedal locomotion during 15 min of treadmill exercise. At each velocity tested (0-06, 0-11 and O'^kmh") , oxygen consumption (V02) showed only a modest, sluggish elevation; a 'steady-state' was never attained. The highest V02 recorded, O-ZZmlOzg"^", was 4-4 times the resting rate. Net whole body lactate (WBL) was found to increase at a constant ...

Abstract Aerobic carboxydotrophic Knallgasbacteria exhibit the unique feature to utilize CO, CO 2 and H or mixtures thereof as sole carbon energy sources in presence of O . While several industrial off-gases are comprised such gases, this group organisms is promising establish novel value chains via gas fermentation for sustainable production chemicals fuels.

Electrodialysis, an electrochemical membrane technique, was found to prolong and enhance the production of biohydrogen and purified organic acids via the anaerobic fermentation of glucose by Escherichia coli. Through the design of a model electrodialysis medium using cationic buffer, pH was precisely controlled electrokinetically, i.e. by the regulated extraction of acidic products with coulomb...

The aim of present study was to assess the effects of individual various essential plant oils on chemical composition, aerobic stability and fermentation characteristics of corn silage and in vitro rumen fermentation and methane emission using gas production technique. Whole crop corn was harvested and ensiled in laboratory scale tubes (4±0.25 kg) for 45 days. Treatments consisted of either cor...