Direct experimental evidence implicates telomere erosion as a primary cause of cellular senescence. Using a well characterized model system for breast cancer, we define here the molecular and cellular consequences of adriamycin treatment in breast tumor cells. Cells acutely exposed to adriamycin exhibited an increase in p53 activity, a decline in telomerase activity, and a dramatic increase in ...

Protective efficacy of DL-alpha lipoic acid on adriamycin induced hepatotoxicity was evaluated in rats. Adriamycin toxicity, induced by a single injection (ip; 15 mg/kg body wt), was expressed by an elevation in alanine transaminase, aspartate transaminase, bilirubin levels in serum and alkaline phosphatase, lactate dehydrogenase, alanine transaminase, aspartate transaminase activity in hepatic...

Adriamycin (ADR) is an important chemotherapeutic agent frequently used in treatment of breast cancer. However, resistance to ADR results in treatment failure in many patients. Recent studies have indicated that microRNAs (miRNAs) may play an important role in such drug-resistance. In the present study, microRNA-452 (miR-452) was found to be significantly down-regulated in adriamycin-resistant ...

Cultured human lymphoma cells exposed to adriamycin exhibited extreme sensitivity to the drug. When these cells were exposed to adriamycin for 12 hr, a 5-log kill in terms of reproductive capacity was observed. Furthermore, under these conditions, adriamycin exerted an immediate (interphase) lethal effect on about 60% of the cells. Synchronized cells revealed that the S-G2 boundary region was t...

Cultured human lymphoma cells exposed to adriamycin exhibited extreme sensitivity to the drug. When these cells were exposed to adriamycin for 12 hr, a 5-log kill in terms of reproductive capacity was observed. Furthermore, under these conditions, adriamycin exerted an immediate (interphase) lethal effect on about 60% of the cells. Synchronized cells revealed that the S-G2 boundary region was t...

The pharmacological and therapeutic effects of Adriamycin entrapped in positively charged and negatively charged lipo somes were compared with those of free Adriamycin in mice. Liposomes were composed of phosphatidylcholine and choles terol mixed with stearyl amine (positive charge) or phosphatidylserine (negative charge). Positive liposomes with entrapped Adriamycin effectively retarded the in...

Adriamycin has a wide variety of biological actions on susceptible cells, several of which may be integrally involved in cytotoxicity. In this paper, we present evidence that one of the alterations in cell function that occurs in the presence of Adriamycin is an elevation in the production of diacylglycerol. The effect is rapid, reaches a peak within 10 min of exposure of Sarcoma 180 cells to A...

Previous studies with Adriamycin-sensitive and -resistant (ADRR) MCF-7 human breast tumor cell lines indicated that Adriamycin formed significantly less hydroxyl radical (.OH) as the result of enhanced detoxification of reactive oxygen intermediates in the ADRR cell line. In order to further define the sites of drug activation and the role of detoxification mechanisms in free radical levels, su...

The anticancer drug adriamycin binds iron and these complexes cycle to reduce molecular oxygen (Zweier, J. L. (1984) J. Biol. Chem. 259, 6056-6058). Optical absorption, EPR, and Mössbauer spectroscopic data are correlated with polarographic O2 consumption and chemical Fe2+ extraction measurements in order to characterize each step in this cycle. Fe3+ binds to adriamycin at physiologic pH formin...

Resistance to chemotherapy remains a major obstacle for the treatment of breast cancer. Understanding the molecular mechanism(s) of resistance is crucial for the development of new effective therapies to treat this disease. This study examines the putative role of p130(Cas) (Cas) in resistance to the cytotoxic agent Adriamycin. High expression of Cas in primary breast tumors is associated with ...