OBJECTIVE To investigate determinants of asymmetric dimethylarginine (ADMA) and symmetric dimethylarginine (SDMA), including single nucleotide polymorphisms (SNPs), in the DDAH1, DDAH2, and AGXT2 genes and their associations with prevalent and incident cardiovascular disease (CVD) in older adults with type 2 diabetes mellitus. RESEARCH DESIGN AND METHODS Prevalent CVD was assessed in men and ...

A procedure is described for the direct determination of methyl derivatives of basic amino acids in total acid hydrolyzates of proteins by the use of a Beckman amino acid analyzer operated in the high sensitivity mode with automatic integration. The conditions described permit the separation of ornithine, lysine, 5-hydroxylysine, N’-monomethyllysine, N”-dimethyllysine, histidine, N’-methylhisti...

BACKGROUND Symmetric dimethylarginine (SDMA) is a small molecule formed by methylation of arginine, and released into blood during protein degradation. SDMA is primarily eliminated by renal excretion and is a promising endogenous marker of glomerular filtration rate (GFR). OBJECTIVES To validate an assay for SDMA measurement, determine stability of SDMA in blood, and compare SDMA with serum c...

AIM Increased levels of asymmetric dimethylarginine (ADMA), an endogenous inhibitor of nitric oxide synthase, have been observed in patients with cardiovascular risk factors and atherosclerosis and in patients with a history of stroke. The role of ADMA and its analogue symmetric dimethylarginine (SDMA) in acute ischemic stroke is yet unclear. We hypothesized that plasma dimethylarginine levels ...

Endogenously produced inhibitors of nitric oxide (NO) synthase, in particular asymmetric dimethylarginine (ADMA), are currently considered of importance in various disease states characterized by reduced NO availability. We investigated the association between plasma levels of ADMA, symmetric dimethylarginine (SDMA), L-arginine, and citrulline and perinatal factors and outcome in 130 preterm (g...

Full-length human protein arginine methyltransferase 7 (PRMT7) expressed as a fusion protein in Escherichia coli was initially found to generate only ω-N(G)-monomethylated arginine residues in small peptides, suggesting that it is a type III enzyme. A later study, however, characterized fusion proteins of PRMT7 expressed in bacterial and mammalian cells as a type II/type I enzyme, capable of pr...

EFFECT OF DIMETHYLARGININE DIMETHYLAMINOHYDROLASE IN TEDEVELOPMENT OF SALT SENSITIVITYbySAMAR ABDULLA NASSERMay 2011Advisor: Dr. John FlackMajor: PhysiologyDegree: Doctor of PhilosophySalt sensitivity is associated with a rise in blood pressure (BP) occurring duringsodium loading and/or a fall in BP during sodium restriction that exceeds randomfluctua...

The enzyme dimethylarginine dimethylaminohydrolase (DDAH) metabolizes asymmetrically methylated arginine (namely L-N monomethylarginine [L-NMMA] and asymmetrical dimethylarginine [ADMA]) residues to citrulline and methylamine.1 Considerable interest has been focused on the biology of DDAH after the discovery that asymmetrically methylated arginines (in particular ADMA) are competitive inhibitor...

The asymmetric methylarginines inhibit nitric oxide synthesis in vivo by competing with L-arginine at the active site of nitric oxide synthase. High circulating levels of asymmetric dimethylarginine predict adverse outcomes, specifically vascular events but there is now increasing experimental and epidemiological evidence that these molecules, and the enzymes that regulate this pathway, play a ...

Protein arginine methylation is catalyzed by a family of enzymes called protein arginine methyltransferases (PRMTs). Three forms of methylarginine have been identified in eukaryotes: monomethylarginine (l-NMMA), asymmetric dimethylarginine (ADMA), and symmetric dimethylarginine (SDMA), all characterized by methylation of one or both guanidine nitrogen atoms of arginine. l-NMMA and ADMA, but not...