We have developed a quick, simple technique for the fluorescent staining of bacterial endospores. This technique was developed using Bacillus subtilis endospores. Our technique requires minimal sample heating so it can safely be carried out entirely in a glove-box. The endospores stain diagnostically as an oval with a standard fluorescent nucleotide stain, acridine orange.

More than 300 million routine clinical analyses are performed annually in the United States. Methods for routine clinical urine examination, including detection of bacteriuria, are briefly reviewed. Prospects of some newer, better techniques to carry out such analyses are introduced. A preliminary report is presented on the use of supravital microscopic fluorescence technique (SMFT), employing ...

To determine whether acridine orange (AO) staining of blood cultures could be used as a substitute for blind subculture when used in conjunction with the BACTEC system (Johnston Laboratories, Inc., Towson, Md.), the two methods were compared on all BACTEC-negative specimens. Since blind subcultures were routinely performed in our laboratory on days 2 and 6 of incubation, AO staining was also pe...

with Acridine Orange By DAvID M. JOHNSON, PEYTON T. PIiArr AND PEnnY G. RuGBY I HE IMPORTANCE OF lymphocytic RNA in immune function has received widespread attention in recent years.’ A case in point is the work of Mannick and Egdahl showing that adoptive immunity can be transferred from one animal to another by means of lymphocytic RNA.2 The RNA of the lymphocyte can be evaluated and compared ...

because of antimicrobial, antioxidant, and anticancer potential, salvia chorassanica bunge (lamiaceae) has been considered as a popular herb in iranian traditional medicine. previous studies have shown remarkable cytotoxic properties of the methanol, n -hexane and dichloromethane extract of s. chorassanica on human cervical cancer cells. to seek the therapeutic potentials of s. chorassanica, th...

In the title compound, C(19)H(15)NO(4), the acridine system is essentially planar (r.m.s. deviation = 0.015 Å). The crystal packing exhibits π-π inter-actions between pairs of centrosymmetric mol-ecules, one of them between the central heterocyclic rings and others between the outer benzene rings of the acridine systems, with centroid-centroid distances of 3.692 (1) and 3.754 (1) Å, respectivel...

Objective—To examine the relative adherence of Candida albicans to oral epithelial cells diVerentiated by Papanicolaou staining. Methods—Oral epithelial cells were collected from 10 healthy adults (five male, five female) and counted. Equal volumes of oral epithelial cells and candida were mixed and incubated. The epithelial cells from this mix were collected by filtration through 10 μm polycar...