The present study was conducted for the optimization of pretreatment process that was used for enzymatic hydrolysis of lignocellulosic biomass (Water Hyacinth, WH), which is a renewable resource for the production of bioethanol with decentralized availability. Response surface methodology has been employed for the optimization of temperature ((o)C), time (hr) and different concentrations of mal...

The importance of an accurate and convenient method of amino acid analysis for proteins and peptides is becoming increasingly obvious with the recent developments in biotechnology and protein engineering. The amino acid analyzer based on ion-exchange chromatography developed by Moore and Stein’,’ has remained the method of choice. The most commonly used conventional protocol of Hirs et aL3 util...

Hydrolysis is a reaction to produce sugars from lignocellulosic raw materials for biochemical production. The present study elucidates the hydrolysis of cellulose and formation of glucose decomposition products catalyzed by 5% to 20% (w/w) formic acid at 180 to 220 °C with an initial cellulose concentration of 10 to 100 g/L. Microcrystalline cellulose was used as a model compound. The experimen...

The feasibility of the combination of fungal with mild acid pretreatments of Glycyrrhiza uralensis residue (GUR) for enzymatic hydrolysis and oil production was studied. Combined pretreatment with Phanerochaete chrysosporium and 2.5% sulfuric acid was shown to be more effective than the acid-only pretreatment. Subsequently, the residue obtained from acid hydrolysis was subjected to enzymatic hy...

The separation method and some properties of isodynamic acid phosphatases catalyzing FMN hydrolysis in spinach leaves were investigated. 1. The enzyme catalyzing FMN hydrolysis was found to occur practically and exclusively in the final supernatant fraction by differential centrifugation. 2. Acid phosphatase catalyzing FMN hydrolysis was separated into three fractions by chromatography on Sepha...

Sir: In a recent communication1* from this laboratory it was reported that in addition to L-threoand D-erythro-a, /?-diaminobutyric acids some yet unidentified basic amino acids also occur in the acid hydrolysates of amphomycin2). These minor constituents were provisionally designated according to their relative elution times (in minutes counting from the ammonia peak) on the short column of th...