Lipoxygenase was purified homogeneously from cups of Pleurotus ostreatus by Sephacryl S-400 HR gel filtration, Dyematrex Green A affinity, and DEAE-Toyopearl 650M ion-exchange chromatographies. The molecular weight of the enzyme was estimated to be 67,000 by sodium dodecyl sulfate-polyacrylamide gel electrophoresis and 66,000 by gel filtration; the isoelectric point was pH 5.1. The optimum pH a...

although several methods have been developed to make gamma-aminobutyric acid (gaba) accumulate in tea, the active ingredients and health benefits vary widely depending on the manufacturing process of tea products. in this study, the levels of bioactive compounds and antioxidant activity in tea leaves given the continuous anaerobic condition were investigated. gaba and other free amino acids suc...

Background: Ascorbate is often applied to enhance stability and robustness of brine-injected pork chops sold for retail, but may affect protein oxidation, while plant extracts are potential substitutes. Methods: Brine-injected pork chops (weight-gain ~12%, NaCl ~0.9%) prepared with ascorbate (225 ppm), green tea extract (25 ppm gallic acid equivalents (GAE)), or maté extract (25 ppm GAE) stored...

provements in the determination of iron by the Nitroso-R Salt Method. Ind. Eng. Chem., Anal. Ed. 16: 272. 1944. 25. SIDERIS, C. P. and YOUNG, H. Y. Effects of iron on chlorophyllous pigments, ascorbic acid, acidity and carbohydrates of Ananas comosus (L.) Merr. supplied with nitrate or ammonium salts. Plant Physiol. 19: 52-75. 1944. 26. SIDERIS, C. P. and YOUNG, H. Y. Effects of potassium on ch...

In this study, walnut green husk extract, which is a valuable waste of horticultural products, was used to enhance antioxidant properties and reduce microbial load of ketchup sauce. Extraction efficiency of walnut green husk extract 22.01%, total phenolic compounds 65.40 (mg gallic acid per 100 g dry husk), flavonoids 10.06 (catechin mg per 100 g dry husk), and EC50 0.17 (mg/mL) were expressed....

For the isolation of blue-green algae from soil and fresh water, temperature is a selective factor of major importance. In a nutritionally non-selective mineral medium, which at 25" supported growth of blue-green algae and of many eucaryotic algae, the development of eucaryotic algae was almost completely suppressed by incubation at 35". Both the number and variety of blue-green algae recoverab...

A sunflower (Helianthus annuus L.) mutant was observed in the progeny of a cross between the sunflower cultivar HA 89 and an amphiploid of a H. divaricatus L. × P21 cross that exhibited loss of dormancy induction in the developing embryo. Seeds of this mutant frequently germinate on the head about 40 d after pollination (DAP). In contrast to other nondormant sunflower mutants reported previousl...

Cat # Product Name Unit Size S-7563 SYBR Green I nucleic acid gel stain *10,000X concentrate in DMSO* ............................................................................................................... 500 μL S-7567 SYBR Green I nucleic acid gel stain *10,000X concentrate in DMSO* ..........................................................................................................

nano silica sulfuric acid as a solid acid, was described to be an effective catalyst for one-pot three-component reaction of kojic acid, aryl aldehydes and indoles for the preparation of 2-substituted aryl (indolyl) kojic acid derivatives. the catalyst was prepared by combination of chlorosulfonic acid to nano silica gel. the size of nanoparticles were observed with sem.all prepared compounds w...

Study on microbial diversity in the unexplored rhizosphere is important to understand their community structure, biology and ecological interaction with the host plant. This research assessed the genetic and functional diversity of fluorescent pseudomonads [FP] in the green gram rhizophere. One hundred and twenty types of morphologically distinct fluorescent pseudomonads were isolated during ve...