The receptors on neuronal membranes for N-methylD-aSpartatf3 (NMDA), an analog of L-glutamic acid, are the focus of intensive study because of their importance in many neurophysiological and neuropathological states. Since there is very little knowledge of the molecular characteristics of the NMDA receptors, we undertook the development of methods for the solubilization and purification of prot...

Granulated blast furnace slag (GGBFS) is a potential resource of rare earth elements (REEs), and due to the complex mineralogy, extraction by conventional hydrometallurgical process makes it an acid-consuming method. Bioleaching thus investigated using chemo-organotrophic bacterium Gluconobacter oxydans (DSMZ 46616) for REE from GGBFS containing 157 ppm Ce, 90 La, 71 Nd 40 Er, hosted in Ca-Al-S...

Separations of proteins at acid pH in the presence of a high concentration of surfactant [sodium laurylsulfate (SDS), 50 mmol/l] was investigated. The purpose of using high concentrations of SDS as background electrolyte modifier was threefold: First, the surfactant exerts a washing effect upon the capillary wall thus preventing binding of analytes and possible clogging of the capillary. Second...

We cloned, expressed, and purified the hdeB gene product, which belongs to the hdeAB acid stress operon. We extracted HdeB from bacteria by the osmotic-shock procedure and purified it to homogeneity by ion-exchange chromatography and hydroxyapatite chromatography. Its identity was confirmed by mass spectrometry analysis. HdeB has a molecular mass of 10 kDa in sodium dodecyl sulfate-polyacrylami...

White root disease is a significant of cashew caused by Rigidoporus sp. Five endophytic fungal isolates, namely AR31D (Fusarium proliferatum), AR42D (Penicillium citrinum), BR32C (Trichoderma asperellum), VNTB1 (Chaetomium sp.), and EAGS14 (Curvularia lunata), were assessed as the biocontrol agents against in vitro in planta. The research objective was to obtain fungi that effectively con...

Solubilization and encapsulation of fullerenes C60 and C70 by ultralarge hollow micelles formed by rod-coil diblock copolymers and characterization of the resulting self-organized fullerene-block copolymer assemblies are reported. The solubilization capacity of each fullerene in poly(phenylquinoline)-blockpolystyrene (PPQ-PS) micelles in binary solvents, trifluoroacetic acid/dichloromethane, an...

Insufficient nutrient and pest infection are two major predicaments for low crop yield in agriculture. Nematodes, a most infectious parasite pest in plants responsible for major yield loss. Chemical phosphate fertilizer made large reserves of it in soil, but part of it is made available to the plants because of irreversible fixation of it with divalent cation. In the present study, Arthrobotrys...

This study focuses on the spectrofluorimetric behaviour of a pharmaceutically and analytically important molecule 1-naphthoic acid in the presence of various surfactant solutions. Micellar solubilization is a powerful alternative for dissolving hydrophobic compounds in aqueous environment. Fluorescence and absorption spectroscopy are the two techniques used to monitor the micellar solubilizatio...