a b s t r a c tintroduction: craving to methamphetamine is a significant health concern and exposure to methamphetamine cues in laboratory can induce craving. in this study, a task designing procedure for evaluating methamphetamine cue-induced craving in laboratory conditions is examined. methods: first a series of visual cues which could induce craving was identified by 5 discussion sessions b...

Computer simulations constitute the basis of the design and discovery of new drugs. This approach is not only significant with regards to finding new structures, but also for selecting the molecules with the highest probability of being useful in the diagnostic process and treatment of numerous diseases. In our work, we used computational software to analyze 32 new acetylcholinesterase (AChE) i...

In this study neurite outgrowth from cultured hippocampal neurones was increased by addition of acetylcholinesterase acting in a non-cholinergic manner. Only monomeric acetylcholinesterase, a form of acetylcholinesterase dominant in development, increased neurite outgrowth (3-10 U/ml); moreover this effect was not blocked by active site blockers (echothiophate and galanthamine) but was sensitiv...

background and objective: changes of the enzyme acetylcholinesterase are involved in pathogenesis of different nervous disorders including alzheimer’s disease. this research work was conducted to evaluate the inhibitory effect of pistacia lentiscus ethanolic extract on acetylcholinesterase activity. materials and methods: activity of the enzyme acetylcholinesterase (ache) was measured by ellman...

Subcellular fractions of the electric tissue of the main organ of the eel Electrophorus electricus were prepared in sucrose media by differential centrifugation and differential discontinuous gradient centrifugation. The distributions of acetylcholinesterase, cytochrome oxidase, DNA, and protein were determined. The appearance of the fractions was determined by phase contrast microscopy and by ...

Galanthamine, physostigmine and 9-amino-1,2,3,4-tetrahydroacridine (tacrine) were evaluated as inhibitors of human acetylcholinesterase activity from samples of postmortem human brain, fresh brain cortex biopsies and human erythrocytes. Acetylcholinesterase activity was most effectively inhibited in all tissues by physostigmine, followed by tacrine and galanthamine. The respective inhibitor con...

Crude chromaffin secretory vesicles, obtained by differential centrifugation, were further purified on isotonic (Percoll) gradients. The chromaffin vesicle fractions recovered from the gradients contain acetylcholinesterase as well as lysosomal enzymes. With the aid of a subsequent sucrose gradient lysosomal enzymes could be removed from chromaffin vesicle fractions, but not acetylcholinesteras...

factors such as oxidative stress and reduced acetylcholine level have been implicated in alzheimer’s disease (ad) pathology and recently there has been a trend towards natural product research to find potential sources of antioxidants and acetylcholinesterase inhibitors in the plants kingdom. centaurea is a genus with about 500 species world wild, many of them have shown to possess biologic act...

Background and Objective: Changes of the enzyme acetylcholinesterase are involved in pathogenesis of different nervous disorders including Alzheimer’s disease. This research work was conducted to evaluate the inhibitory effect of Pistacia lentiscus ethanolic extract on acetylcholinesterase activity. Materials and Methods: Activity of the enzyme acetylcholinesterase (AChE) was measured by Ellman...

In the symptomatic treatment of mild to moderately severe dementia associated with Alzheimer’s disease, donepezil (E2020) has been introduced for the inhibition of acetylcholinesterase activity in the human brain. However, there is no morphological evidence as to how this chemical agent affects the acetylcholinesterase-positive structures in the various areas of the human and the rat CNS. This ...