the families of tgf-β proteins are the most important growth factors in the ovary for growth and differentiation of early ovarian follicles. three related oocyte-derived members of the transforming growth factor-β superfamily namely growth differentiation factor 9 (gdf9), bmp15 and bmpr-ib have been shown to be essential for follicular growth and ovulation. different mutations in the gdf9 gene ...

The present investigation was to study DNA polymorphism of insulin-like growth factor binding protein-3 (IGFBP-3) gene by PCR-RFLP in four Egyptian local sheep breeds. Genomic DNA was isolated from a total of 20 animals from four Egyptian breeds (five each) of sheep namely Rahmani, Ossimi, Awassi, and Barki. A fragment of IGFBP-3 gene, comprising of a part of exon 2, complete intron 2, exon 3, ...

Booroola gene is one of the major genes in the enhancement of ovulation rate and could be an attractive candidate gene for ovulation rate in sheep. Molecular technologies have been widely used for discovery of mutations in animal species. These mutations have major effects on the important economic traits of sheep and goats. This study was conducted to identify the mutation in FecXG region of e...

Given the ecological and epidemiological specialization of Hyalesthes obsoletus, principle vector ‘Candidatus Phytoplasma solani’, primary objective this study was to develop molecular tools for discriminating three host plant associations populations: (i) Convolvulus arvensis–Urtica dioica (Ca–Ud), (ii) Vitex agnus-castus (Vac), (iii) Crepis foetida (Cf). The genetic diversity nearly full-leng...

The use of invertebrate-derived DNA (iDNA) is a promising non-invasive tool to monitor wildlife. While most studies have been carried out in dense tropical and sub-tropical forests focused on the single category invertebrates, this study compares flies mosquitoes-derived assess vertebrate diversity semi-urban environments. We conducted our sampling four different forest plots Berlin, Germany. P...

objective the aim of this study was to compare the sensitivity and specificity of acid fast and auramine-rhodamine staining and multiplex pcr for the detection of mycobacterium tuberculosiscomplex and non tuberculosis mycobacteria on formalin fixed paraffin embedded tissues (ffpe) materials and methods forty cases of ffpe pleural and bronchial tissue with chronic granulomatous inflammation and ...

ABSTRACT: Streptomyces sampsonii is a kind of biocontrol bacterium with antifungal effects, and chitinase one the main substances. To improve further study structure function gene S. sampsonii, we amplified target fragment by PCR, ligated to expression vector pET-32a, introduced resulting plasmid into Escherichia coli BL21 (DE3) induced chitinase. Then, recombinant was purified is-labelled prot...

introduction: toxoplasma gondii is a protozoan parasite which is globally prevalent in human and animals. toxoplasma infection is commonly asymptomatic, but can cause serious medical problems in immunocompromised individuals and in fetus. recombinant antigens of the parasite may be helpful in diagnosing the infection more precisely. the goal of this study was to construct and evaluate the funct...