The biochemical properties of a purified enzyme of a new alkalophillic endo-1,4-beta-xylanase gene, KRICT PX2 (GU967374), which was isolated from Paenibacillus sp. HPL-002 (KCTC11410BP) and expressed in E. coli, were investigated. The specific activity of the purified xylanase was 51.26 μmol/min/mg proteins. The Km and Vmax values of the protein for birch wood xylan were also verified to have 0...

Solid-state fermentation (SSF) was carried out to produce α-amylase from Aspergillus oryzae (IFO 30103) using spent brewing grains (SBG) as substrate. A maximum of 11296 U/gds amylase activity was obtained after 48 h of fermentation. The extracted enzyme was subjected to partial purification by ammonium sulphate fractionation. Maximum specific activity was obtained with 40-70% fraction. SDS-PAG...

Mild Steel and their products are main raw material for a strong infrastructure for every country which is the main demand in the race of survival, stabilization, growth and competition for every country. Industrialization and modernization in now a days has made a strong demand of steel and their maintenance but atmospheric corrosion can aggressively accelerate the degradation of metals during...

The effects of caffeine (0.2-10 mM) on the electrical and mechanical activities of guinea pig taenia coli were investigated with the double sucrose-gap method. Caffeine evoked a small tension with a latency of 20-30 sec, then phasic contraction developed and finally relaxation. The initial tension development also appeared in the Na-free solution without any marked changes in the membrane poten...

Production of αamylase under solid state fermentation by Streptomyces erumpens MTCC 7317 was investigated using cassava fibrous residue, one of the major solid waste released during extraction of starch from cassava (Manihot esculenta Crantz). Response surface methodology (RSM) was used to evaluate the effect of the main variables, i.e., incubation period (60 h), moisture holding capacity (60%)...

Nanomaterials are extensively studied in electrochemical energy storage and conversion systems because of their structural advantages. However, their volumetric energy density still needs improvement due to the high surface area, especially the carbon based nanocomposites. Constructing hierarchical micro-scaled materials from closely stacked subunits is proposed as an effective way to solve the...

The extremely halophilic archaeon Natrinema sp. strain SSBJUP-1, which produces extracellular cellulase and xylanase, was isolated from the Lonar Lake situated in Buldhana, Maharashtra. The enzymes were optimally active at pH 9–10 and temperature 40–60 C and they were most stable up to pH 11 and 16 % of NaCl concentration. The xylanase showed maximum activity at 50C and cellulase at 40C. Xylana...

Partial hydrolysis of whey proteins by enzymes immobilized on an inert support can either change or evidence functional properties of the produced peptides, thereby increasing their applications. The hydrolysis of sweet cheese whey proteins by alcalase, which is multipoint-immobilized on agarose gel, is studied here. A MichaelisMenten model that takes into account competitive inhibition by the ...

The aim of current study was to determine intercropping forage turnip “FT” with cereals “C” (barley, “B”, wheat “W” and oat “O”) for herbage yield competitive ratios in Bilecik conditions 2019-2002 2020-2021 growing periods. Plants were sown as sole 3 different mixtures (75FT+25C%, 50FT+50C% 25FT+75C %). Experiments arranged a randomized complete block design three replications. In the study, y...

Abstract Background Cabotegravir + Rilpivirine Long Acting (CAB+RPV LA) every 2 months is a recommended regimen in European and US treatment guidelines for PLWH with virological suppression no known resistance to CAB or RPV. CARISEL, an implementation study, the first study where all participants switched from standard oral therapy monthly CAB+RPV. Key clinical secondary endpoints are reported....