Abstract Corydalis saxicola Bunting, a medicinal herb known for alkaloids, grows mainly in Guizhou province and northwest Guangxi of China. In this study, the first time plant, an efficient adventitious shoot proliferation regeneration system via organogenesis from leaf petiole explants was established. Cytokinins, including 6-benzyladenine (BA) thidiazuron (TDZ), induced callus then organogene...

Synthetic seed technology is an alternative to traditional micropropagation for production and delivery of cloned plantlets. Synthetic seeds were produced by encapsulating nodal segments of C. angustifolia in calcium alginate gel. 3% (w/v) sodium alginate and 100 mM CaCl2 · 2H2O were found most suitable for encapsulation of nodal segments. Synthetic seeds cultured on half strength Murashige and...

Antibacterial activity of Costus igneus was conducted. The plant C. igneus was well known for its anti-diabetic property. Traditionally the plant was used to treat fever, rash, asthma and intestinal worms. Two growth regulator IAA (Indole 3-acetic acid) and IBA (Indole butyric acid) in combinations were applied to MS medium for direct root induction. MS medium supplemented with 0.5mg/ml IBA, in...

the growing demand for animal products has made importance of the utilization of biotechnological techniques in improvement of forage crops such as sainfoin. in this research, the effects of different levels of auxin (naa and iba) and cytokinin (bap, zeatin and tdz) on in vitro growth response of shoot apex explants of shahrkord and khansar cultivars of sainfoin were investigated. for this, sho...

in order to study, in vitro bud induction and shoot regeneration of different explants (shoot tip, axillary bud, hypocotyl and cotyledon) of henbane on ms medium enriched with various concentrations of kinetin (0, 1, 3 and 5 mg.l-1) alone or in combination with indole-3 acetic acid (0, 0.1 and 0.5 mg.l-1) this research was investigated in urom zist tak knowledge-based company plant tissue cultu...

the jatropha curcas l., a plant belongs to euphorbiaceae family, which has great economic and medicinally value. there is high amount of oil in seeds of this plant. because of low germination ability of seed, propagation of jatropha curcas via seed is not usual. the purpose of this work is optimizing of jatropha curcas tissue culture for optimization of callus formation and propagation through ...

a methodology to regenerate whole plants of fig tree (ficus carica cv. jaami-e-kan) from apical meristem of greenhouse plantlets was developed. two meristematic explants size (0.2-0.4 and 0.5-0.7 mm), two kinds of culture media and three concentrations of 6-benzyladenine (0.5, 1 and 1.5 mgrl-1) were tested as a completely randomize design (crd) with a simple factorial arrangement for establishm...

The present investigation was carried out to study the In vitro shoot multiplication and callogenesis in Gladiolus. For this purpose, cormel, meristem and leaf explants were chosen. Different concentrations of BAP i. e., 0.5, 1.0, 1.5, 2.0 or 2.5 mgL (2.22, 4.44, 6.66, 8.88 or 11.10 μM) were supplemented to MS medium. Both cormel and meristem explants showed maximum multiplication on MS medium ...

The aim of this investigation was micropropagation of Quercus brantii L. via culture of terminal and lateral buds for mass production of this plant. The plant materials were collected from Fars province Iran in spring and summer seasons. After surface sterilization and the removal of phenol materials from the explants, they were transferred into different culture media containing different conc...